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Authentic L-Rha standard. Serotype 7 GPL. 23.00. 100. 22.60. 100. ( ± )-2-butyl. 80. 23.00. 80. 60. 60. 40. 40. 20. 20. 0. 0. 0. 5. 10. 15. 20. 25. 30. 35. 40. 0. 5. 10. 15. 20. 25. 30. 35. 40. Time (min). Time (min). R-(-)-2-butyl. R-(-)-2-butyl. L-Rha.
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Authentic L-Rha standard Serotype 7 GPL 23.00 100 22.60 100 (±)-2-butyl 80 23.00 80 60 60 40 40 20 20 0 0 0 5 10 15 20 25 30 35 40 0 5 10 15 20 25 30 35 40 Time (min) Time (min) R-(-)-2-butyl R-(-)-2-butyl L-Rha L-Rha 22.60 100 80 60 40 20 0 22.60 0 5 10 15 20 25 30 35 40 100 S(+)-2-buty S(+)-2-butyl Time (min) L-Rha L-Rha 80 22.99 60 100 80 40 60 20 40 0 20 0 5 10 15 20 25 30 35 40 Time (min) 0 0 5 10 15 20 25 30 35 40 Time (min) Supplement Fig. 1 Comparative GC analysis of (R)-(-)-2-butyl and (S)-(+)-2-butyl glycosides prepared from an authentic L-Rha standard and serotype 7 GPL.