1 / 12

Soil Microbiology Group

Soil Microbiology Group. Soil Microbial communities from Puerto Rico rain forest (that decompose switchgrass , sample from Feedstock-adapted consortia SG and SG+Fe ). Alinne Castro – University of Brasilia Jessica C. Bergmann – JBEI

luisa
Télécharger la présentation

Soil Microbiology Group

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Soil Microbiology Group Soil Microbial communities from Puerto Rico rain forest (that decompose switchgrass, sample from Feedstock-adapted consortia SG and SG+Fe) Alinne Castro – University of Brasilia Jessica C. Bergmann – JBEI VaraKensakurti Samba Si – Agriculture & Agri-Food Canada

  2. Overview • Introduction • Where this soil came from? • What is the difference between them? • Comparison of the two samples • What are the populations in the two metagenomes? • Are the two populations functionally different from each other? • Is there any Glycosyl Hydrolases (GHs) in these samples?

  3. Where this soils came from? • Environment: Soil from Puerto Rico Rain Forest (Luquillo) • Soil collected April 2010 • Sequenced in 454 GS-FLX-Titanium

  4. Samples – Gene Count

  5. What is the difference between the two soils? • SG – Switchgrass (carbon source) • SG + Fe – Switchgrass enriched with iron • Iron addition – increase diversity of feedstock – cultures limited for electron donor

  6. Overview • Introduction • Where this soil came from? • What is the difference between them? • Comparison of the two samples • What are the populations in the two metagenomes? • Are the two populations functionally different from each other? • Is there any Glycosyl Hydrolases (GHs) in these samples?

  7. What are the populations in the two metagenomes? • Compare Genomes (Metagenome X Genome) • Percent Identity – 90% - Phylum SG SG + Fe

  8. Order SG SG + Fe • Compare Genomes – Phylogenic Distribution - Radial Tree

  9. Functional Difference 54 significantPfamfunction • Analysis Cart – Profile and Alignment – View Function vs. Genome

  10. Is there any Glycosyl Hydrolases (GHs) in these samples?

  11. Is there any Glycosyl Hydrolases (GHs) in these samples? • IMG – Find Function – Save to Function Cart – Profile and Alignment Tool

  12. Thank you !

More Related