EPITHELIA & MESENCHYME

1. EPITHELIA & MESENCHYME This resource is licensed under the Creative Commons Attribution Non-Commercial & No Derivative Works License

2. Objectives • Identify epithelia and mesenchyme • Identify the neural tube, notochord and somites • in tissue sections and the types of cells they • are composed of • Relate what you see to events that happen in • the embryo and to adult anatomy

3. SLIDE A : Early embryonic chick, TS section Identify : neural groove, mesenchyme, ectoderm, endoderm, hypoblast and site of notochord 250µm

4. SLIDE A : Early embryonic chick, TS section Identify : neural groove, mesenchyme, ectoderm, endoderm, hypoblast and site of notochord neural groove ectoderm mesenchyme of mesodermal origin condensing notochord hypoblast endoderm 250µm

5. SLIDE A : Early embryonic chick, TS section Identified on a similar section of Slide A: neural groove, mesenchyme, ectoderm, endoderm, hypoblast and site of notochord neural groove ectoderm mesenchyme of mesodermal origin condensing notochord endoderm 250µm

6. SLIDE A : Early embryonic chick, TS section neural groove ectoderm mesenchyme of mesodermal origin 50µm condensing notochord endoderm

7. SLIDE B : Early embryonic chick, TS section Identify : neural plate, mesoderm, ectoderm, endoderm, notochord 100µm

8. SLIDE B : Early embryonic chick, TS section Identify : neural plate, mesoderm, ectoderm, endoderm, notochord artefact ectoderm neural plate mesoderm endoderm condensing notochord 100µm

9. SLIDE B : Early embryonic chick, TS section Identify : neural plate, mesoderm, ectoderm, endoderm, notochord 100µm

10. SLIDE B : Early embryonic chick, TS section Identify : neural plate, mesoderm, ectoderm, endoderm, notochord surface ectoderm neural plate/tube mesoderm mesoderm mesoderm mesoderm endoderm notochord 100µm

11. SLIDE B : Early embryonic chick, TS section surface ectoderm neural plate/tube mesoderm notochord endoderm 50µm

12. SLIDE C : Embryonic cat, TS section Identify : neural tube, notochord, somites, coelom, dorsal aorta, somatopleure, splanchnopleure and identify the uterine wall 0.5 mm

13. SLIDE C : Embryonic cat, TS section Identify : neural tube, notochord, somites, coelom, dorsal aorta, somatopleure, splanchnopleure and identify the uterine wall uterine wall somatopleure neural tube somite coelom splanchnopleure dorsal aorta paired notochord 0.5 mm

14. SLIDE C : Embryonic cat, TS section Identify : neural tube, notochord, somites, coelom, dorsal aorta, somatopleure, splanchnopleure also intermediate mesoderm, somatic mesoderm,somatic ectoderm, splanchnic mesoderm, splanchnic endoderm and the dermatome, myotome and sclerotome of the somites 250µm

15. SLIDE C : Embryonic cat, TS section D: dermatome M: myotome S: sclerotome IM: intermediate mesoderm DA: dorsal aorta neural tube surface ectoderm somatopleure somatic ectoderm somatic mesoderm D M coelom S IM splanchnic mesoderm DA DA splanchnopleure endoderm notochord 250µm

16. SLIDE C : Embryonic cat, TS section D: dermatome M: myotome S: sclerotome IM: intermediate mesoderm neural tube 50µm DA: dorsal aorta surface ectoderm somatic ectoderm D somatopleure M somatic mesoderm S splanchnic mesoderm coelom IM DA DA notochord splanchnopleure endoderm

17. SLIDE 129 : Rabbit foetus (20 day) • First of all orientate yourself, as this is a complicated section containing many different tissues. • Identify structures seen in slides A,B and C. • What other major organs can you identify? 1.0 cm

18. SLIDE 129 : Rabbit foetus (20 day) 1. brain 2. nasal cavity 3. oral cavity 4. palate 5. tongue 6. vertebral column 7. sections through limbs 8. ribs 9. heart 10. lung 11. diaphragm 12. liver 13. intestine 6 6 10 13 11 9 12 1 3 5 8 4 2 7 7 1.0 cm

19. Lectures Dr Imelda McGonnell, Dr Claire Russell, Dr Tej Dhoot Histology. 026. Epithelia & mesenchyme J.Bredl. 14-09-2010 Histology Slides and Stains. Tanya Hopcroft.