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Lab Exercise 2

Lab Exercise 2. Culture Media. General Purpose Media: Nutrient Broth and Nutrient Agar. Enriched Media. Selective Media: Mycological agar. a. b. c. Differential Media. Glucose Fermentation a. Neutral pH uninoculated tube of glucose broth with phenol red pH indicator.

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Lab Exercise 2

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  1. Lab Exercise 2 Culture Media

  2. General Purpose Media: Nutrient Broth and Nutrient Agar

  3. Enriched Media

  4. Selective Media: Mycological agar

  5. a b c Differential Media Glucose Fermentation a. Neutral pH uninoculated tube of glucose broth with phenol red pH indicator. b. Fermentation of glucose and acid production (color change from red to yellow) (acidic pH) c. Organism growth, but negative for acid production (alkaline pH)

  6. Differential Media: MacConkey Agar left: no lactose fermentation right: lactose fermentation

  7. Gel media (in-between liquid broth and solid agar) is used to test for motility Spreading out indicates motility

  8. Sterilization by autoclaving: 15-20 psi at 121°C for15-20 minutes

  9. Bunsen burner flame

  10. Aseptically pouring agar plates

  11. Agar plates are stored upside down to prevent condensation.

  12. All labeling is done on the bottom of the agar plate • Initials • 2. Date (mm/dd/yy) • 3. Code # or letter

  13. Environmental sampling

  14. Typical environmental sampling results

  15. Microbes in the Environment. This plate was inoculated with 500 L of indoor air. Approximately how many colonies are on the plate? How many different bacterial species are here? 2 3 4 5 6 1 7 8

  16. Microbes in the Environment. This plate was inoculated with a cotton swab that was wiped over a shower drain. Fungi usually produce large, "fuzzy" colonies (marked F). Approximately how many colonies are on the plate? How many are bacterial colonies? How many different bacterial species are here? F

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