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12192 2012 337 MOESM1 ESM PowerPoint Presentation
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12192 2012 337 MOESM1 ESM

12192 2012 337 MOESM1 ESM

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12192 2012 337 MOESM1 ESM

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  1. Online Resource 1 List of species and NCBI accession numbers used for heat shock factor protein phylogenic and protein domain analysis

  2. Online Resource 2 Sequence of HSE and CBF oligonucleotides used for EMSA. Nucleotides altered from the perfect HSE sequence are underlined. Over-hanging nucleotides used for end-fill labeling are indicated by lower case letters

  3. Online Resource 3 Efficiency of hsf4 gene knockdown using four concentrations of MO. Total numbers of embryos injected with hsf4-MO and hsf4 5bpmm-MO as well as uninjected and danieau injected controls are listed. The percentage of embryo survival 48 hours after injection is also listed. Efficiency of the MO injections in surviving embryos was determined by calculating percentage of embryos that displayed strong flouresceine fluorescence. The number of embryos containing flouresceine and showing non-specific deformities at each MO concentration was tallied to determine the optimal MO concentration for injection