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DNA Ladder for Plasmid Mapping: Analysis and Visualization

Use a DNA ladder to map plasmid DNA, determine cutting sites, and create a visual map. Collect data, measure distances, and graph on a logarithmic scale for accurate analysis. Before starting, heat the ladder at 65C for 2 minutes. Various DNA fragment sizes included in the ladder will help you in this exploration.

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DNA Ladder for Plasmid Mapping: Analysis and Visualization

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  1. DATA ANALYSIS

  2. L 2 3 4 5 6 7 8

  3. DNA Ladder for Mapping of Plasmid DNA: • BEFORE adding ladder heat at 65C for 2 minutes • 23,130 bp • 9416 bp • 6556 bp • 4361 bp • 3000 bp [brighter than other bands!] • 2322 bp • 2027 bp • 725 bp • 570 bp

  4. You will collect data and determine: • Location of cutting sites • Present the “map” of the plasmid in your report

  5. Data will be obtained from your photo DNA Fragments various sizes: Ladder “M”

  6. Standard Curve • Measure distance migrated of known sizes • Versus • DNA known sizes • Graph • Measure distance migrated of unknown

  7. Graph Log Scale: Standard CurveThis is from your ladder DNA (bp) Distance (cm)

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