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Course Project = Algal Lipid Production Decide which algae to study

Course Project = Algal Lipid Production Decide which algae to study http://www.cbs.umn.edu/lab/wackett/links/oil Learn more about cell walls and lipid synthesis Design some experiments See where they lead us. Course Project = Algal Lipid Production Decide which algae to study

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Course Project = Algal Lipid Production Decide which algae to study

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  1. Course Project = Algal Lipid Production • Decide which algae to study • http://www.cbs.umn.edu/lab/wackett/links/oil • Learn more about cell walls and lipid synthesis • Design some experiments • See where they lead us

  2. Course Project = Algal Lipid Production • Decide which algae to study • http://www.cbs.umn.edu/lab/wackett/links/oil • Next assignment: each pick an alga and product and convince the group in 5-10 minutes why your choice is best. Next Wed?

  3. Course Project = Algal Lipid Production • Decide which algae to study • http://www.cbs.umn.edu/lab/wackett/links/oil • Next assignment: each pick an alga and product and convince the group in 5-10 minutes why your choice is best. Next Wed? • Potential experiments • Effects of environment on lipid production

  4. Course Project = Algal Lipid Production • Decide which algae to study • http://www.cbs.umn.edu/lab/wackett/links/oil • Next assignment: each pick an alga and product and convince the group in 5-10 minutes why your choice is best. Next Wed? • Potential experiments • Effects of environment on lipid production • pCO2

  5. Course Project = Algal Lipid Production • Decide which algae to study • http://www.cbs.umn.edu/lab/wackett/links/oil • Next assignment: each pick an alga and product and convince the group in 5-10 minutes why your choice is best. Next Wed? • Potential experiments • Effects of environment on lipid production • pCO2 • temperature

  6. Course Project = Algal Lipid Production • Decide which algae to study • http://www.cbs.umn.edu/lab/wackett/links/oil • Next assignment: each pick an alga and product and convince the group in 5-10 minutes why your choice is best. Next Wed? • Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity

  7. Course Project = Algal Lipid Production • Decide which algae to study • http://www.cbs.umn.edu/lab/wackett/links/oil • Next assignment: each pick an alga and product and convince the group in 5-10 minutes why your choice is best. Next Wed? • Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity • Intensity

  8. Course Project = Algal Lipid Production • Decide which algae to study • http://www.cbs.umn.edu/lab/wackett/links/oil • Next assignment: each pick an alga and product and convince the group in 5-10 minutes why your choice is best. Next Wed? • Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity • Intensity • Duration (= photoperiod=daylength)

  9. Course Project = Algal Lipid Production • Decide which algae to study • http://www.cbs.umn.edu/lab/wackett/links/oil • Next assignment: each pick an alga and product and convince the group in 5-10 minutes why your choice is best. Next Wed? • Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity • Light quality

  10. Course Project = Algal Lipid Production • Decide which algae to study • http://www.cbs.umn.edu/lab/wackett/links/oil • Next assignment: each pick an alga and product and convince the group in 5-10 minutes why your choice is best. Next Wed? • Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity • Light quality = color(s)

  11. Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity • Light quality = color(s) • Nutrition

  12. Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity • Light quality = color(s) • Nutrition • N

  13. Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity • Light quality = color(s) • Nutrition • N • P

  14. Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity • Light quality = color(s) • Nutrition • N • P • K

  15. Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity • Light quality = color(s) • Nutrition • N • P • K • S

  16. Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity • Light quality = color(s) • Nutrition • N • P • K • S • Ca

  17. Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity • Light quality = color(s) • Nutrition • N • P • K • S • Ca • Fe

  18. Potential experiments • Effects of environment on lipid production • pCO2 • Temperature • Light quantity • Light quality = color(s) • Nutrition • N • P • K • S • Ca • Fe • Mg

  19. Potential experiments • Effects of environment on lipid production • Nutrition • N • P • K • S • Ca • Fe • Mg • micronutrients

  20. Potential experiments • Effects of environment on lipid production • Nutrition • N • P • K • S • Ca • Fe • Mg • Micronutrients • Vitamins

  21. WATER • Plants' most important chemical • most often limits productivity • Gives cells shape • Dissolves many chem: most biochem occurs in water • Constantly lose water due to PS (1000 H2O/CO2)

  22. Plant Water Uptake Water is drawn through plants along the SPAC, relying on adhesion & cohesion (&surface tension) to draw water from the soil into the air

  23. Water potential • Water moves to lower its potential • Depends on: • [H2O]: Ys (osmotic potential) • Pressure Yp • GravityYg Yw = Ys +Yp + Yg

  24. Measuring water potential • YP(pressure potential) is hard! • Pressure bomb = • most common technique • Others include pressure • transducers, xylem probes • Therefore disagree about H2O • transport in xylem

  25. Water transport • Therefore disagree about H2O • transport in xylem • Driving force = evaporation • in leaves (evapotranspiration) • Continuous H2O column • from leaf to root draws up • replacement H2O from soil (SPAC)

  26. Water transport • Driving force = evaporation • in leaves (evapotranspiration) • Continuous H2O column • from leaf to root draws up • replacement H2O • Exact mech controversial

  27. Water transport • Driving force = evaporation in leaves (evapotranspiration) • Continuous H2O column from leaf to root draws up • replacement H2O • Exact mech controversial • Path starts at root hairs

  28. Water transport • Path starts at root hairs • Must take water from soil

  29. Measuring water potential • Path starts at root hairs • Must take water from soil • Ease depends on availability • & how tightly it is bound

  30. Measuring water potential • Path starts at root hairs • Must take water from soil • Ease depends on availability & how tightly it is bound • Binding depends on particle size & chem

  31. Measuring water potential • Must take water from soil • Ease depends on availability & how tightly it is bound • Binding depends on particle size & chem • Availability depends on amount in soil pores

  32. Measuring water potential • Availability depends on amount in soil pores • Saturation: completely full

  33. Measuring water potential • Availability depends on amount in soil pores • Saturation: completely full • Field capacity: amount left after gravity has drained excess

  34. Measuring water potential • Availability depends on amount in soil pores • Saturation: completely full • Field capacity: amount left after gravity has drained excess • Permanent wilting point: amount where soil water potential is too negative for plants to take it up

  35. Water movement in plants Water enters via root hairs mainly through apoplast until hits Casparian strip : hydrophobic barrier in cell walls of endodermis

  36. Water movement in plants Water enters via root hairs mainly through apoplast until hits Casparian strip : hydrophobic barrier in cell walls of endodermis Must enter endodermal cell

  37. Water Transport Water enters via root hairs mainly through apoplast until hits Casparian strip : hydrophobic barrier in cell walls of endodermis Must enter endodermal cell Why flooded plants wilt!

  38. Water Transport Water enters via root hairs mainly through apoplast until hits Casparian strip : hydrophobic barrier in cell walls of endodermis Must enter endodermal cell Why flooded plants wilt! Controls solutes

  39. Water Transport Must enter endodermal cell Controls solutes Passes water & nutrients to xylem

  40. Water Transport Passes water & nutrients to xylem Ys of xylem makes root pressure

  41. Water Transport Passes water & nutrients to xylem Ysof xylem makes root pressure Causes guttation: pumping water into shoot

  42. Water Transport Passes water & nutrients to xylem Ysof xylem makes root pressure Causes guttation: pumping water into shoot Most water enters near root tips

  43. Water Transport Most water enters near root tips Xylem is dead! Pipes for moving water from root to shoot

  44. Water Transport Most water enters near root tips Xylem is dead! Pipes for moving water from root to shoot Most movement is bulk flow

  45. Water Transport • Xylem is dead! Pipes for moving water from root to shoot • Most movement is bulk flow • adhesion to cell wall helps

  46. Water Transport • Xylem is dead! Pipes for moving water from root to shoot • Most movement is bulk flow • adhesion to cell wall helps • Especially if column is broken by • cavitation (forms embolisms)

  47. Water Transport • Most movement is bulk flow • adhesion to cell wall helps • Especially if column broken by cavitation • In leaf water passes to mesophyll

  48. Water Transport • Most movement is bulk flow • adhesion to cell wall helps • Especially if column broken by cavitation • In leaf water passes to mesophyll, then to air via stomates

  49. Water Transport • In leaf water passes to mesophyll, then to air via stomates • Driving force = vapor pressure deficit (VPD) • air dryness

  50. Water Transport • In leaf water passes to mesophyll, then to air via stomates • Driving force = vapor pressure deficit (VPD) • air dryness • ∆ H2O vapor pressure [H2O(g)] • & saturated H2O vapor pressure

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