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High Sucrose Soybean Thin-Layer Chromatography TLC

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High Sucrose Soybean Thin-Layer Chromatography TLC

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    1. High Sucrose Soybean Thin-Layer Chromatography (TLC)

    2. High Sucrose Soybean Seed Preparations

    3. High Sucrose Soybean Seed Preparations Step 1 Measure out one cup of soybeans for each variety called for in the protocol. Place the soybeans in a beaker and completely cover with water. Let stand for 18-24 hours.

    4. High Sucrose Soybean Seed Preparations Step 2 Drain the water off the soybeans using a kitchen strainer and rinse with cold tap water. Use the soybeans in the protocol per the directions.

    5. High Sucrose Soybean Thin-Layer Chromatography (TLC) Activity

    6. High Sucrose Soybean TLC Solution Preparation Step 1 Put the soybeans and an equal amount of distilled water in a blender and blend on high for one minute.

    7. High Sucrose Soybean TLC Solution Preparation Step 2 After blending is complete, filter the soybean mixture through two #6 coffee filters placed in a kitchen strainer. Collect the filtrate in a clean beaker. Repeat for each variety of soybean required in protocol.

    8. High Sucrose Soybean TLC Solution Preparation Step 3 Label three 1.5 ml tubes. One with N for normal, one with S for standards, and one with H for high sucrose.

    9. High Sucrose Soybean TLC Solution Preparation Step 4 Each sample is then diluted to 1/5th its original concentration by placing one drop of filtrate from each variety into the appropriate 1.5 ml tube and adding four drops of distilled water.

    10. High Sucrose Soybean TLC TLC Plate Preparation Step 1 Each group should prepare a TLC plate. Make sure to turn the plate white-side-up before starting. Use a ruler to make a very light pencil line across the plate 15 mm from the bottom.

    11. High Sucrose Soybean TLC TLC Plate Preparation Step 2 Then make three very light marks 1 cm from each edge of the plate and one mark 1.5 cm for the first. Label very lightly with pencil the marks from left to right; N, S, and H.

    12. High Sucrose Soybean TLC Activity Step 1 Fill a 10 ul micro-pipette by placing it in the tube marked N. Capillary action will automatically fill the tube.

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