1 / 10

Thin Layer Chromatography

Thin Layer Chromatography. bsapp.com. Preparing a Sample Space. Cut a sheet of paper so it may hang free within the intended container Mark a sample line ½ inch to an inch from the bottom. bsapp.com. Place your samples on the sheets. bsapp.com. Starting the Sample.

wren
Télécharger la présentation

Thin Layer Chromatography

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Thin Layer Chromatography bsapp.com

  2. Preparing a Sample Space • Cut a sheet of paper so it may hang free within the intended container • Mark a sample line ½ inch to an inch from the bottom bsapp.com

  3. Place your samples on the sheets bsapp.com

  4. Starting the Sample • Place the sheet in a container with a solvent • The bottom of the paper should be submerged into the solvent, but not above the samples bsapp.com

  5. Allow your samples to run at least ½ way up the sample space bsapp.com

  6. bsapp.com

  7. Allow your samples to dry bsapp.com

  8. Matching Samples • Note component colors • Calculate Rf values bsapp.com

  9. Calculating Rf Values • Rf value is a ratio of distance a component sample moves to the distance the solvent moves • This number is usually expressed as a decimal bsapp.com

  10. Solvent finish 10 cm Calculating Rf Values • 8 cm • 4 cm Rf = 4/10 = 0.4 Rf = 8/10 = 0.8 • Starting point bsapp.com

More Related