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Gene Expression Analysis of Breast Cancer Cell Lines and Normal Breast Epithelial Cells

This study investigates the expression levels of key genes in various breast cancer cell lines (MDA-MB-231, MCF-7, MCF-10A) and normal human mammary epithelial cells (HMEC). Using PCR amplification across 20, 25, and 29 cycles, we analyze transcripts including CUL-5 (674 bp) and Ribosomal Protein S9 (411 bp). The assay includes a 100 bp ladder for size verification, emphasizing the differences in gene expression profiles among the tested cell lines. The findings contribute to understanding the molecular mechanisms underlying breast cancer progression.

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Gene Expression Analysis of Breast Cancer Cell Lines and Normal Breast Epithelial Cells

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  1. MDA-MB-231 MDA-MB-231 MDA-MB-231 100-bp ladder HMEC MCF-10A MCF-7 NT HMEC MCF-10A MCF-7 NT HMEC MCF-10A MCF-7 NT 20 cycles 25 cycles 29 cycles A. CUL-5 674-bp 20 cycles 25 cycles 29 cycles B. Ribosomal protein S9 411-bp 100-bp ladder HMEC MCF7 MCF-10A MDA-MB-231 EcoR I EcoR I EcoR I EcoR I C. CUL-5 674-bp - - - - + + + +

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