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Conclusions

The Effect of High Concentrations of GA 3 and Seed Age on Germination of Silene latifolia Allison Amore, Meredith Harry, William Wood Department of Biology, Davidson College. Age Decreases Viability. Conclusions

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Conclusions

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  1. The Effect of High Concentrations of GA3 and Seed Age on Germination of Silene latifolia Allison Amore, Meredith Harry, William Wood Department of Biology, Davidson College Age Decreases Viability Conclusions GA3 increases mean fraction germination of seeds independent of seed age or concentration Seeds from 2002 germinated at a higher mean fraction while seeds from 2003 had a higher mean viability GA3 overcame the after-ripening requirement for the 2003 seeds A GA3 concentration of 2.601 X 10-2 M showed no toxicity Future Research Test higher concentrations of GA3 to determine toxicity level Test a population using more years with a greater gap between them Test other populations of white campion Acknowledgements We would like to thank Dr. Patricia Peroni for her valuable assistance and the previous Bio 341 lab group. Question • Do increased concentrations of GA3 and/or seed age affect germination in Silene latifolia? Background • White campion, (Silene latifolia or Silene alba) - dioecious, perennial plant, native to Europe - excellent model species to study the dynamics of a fragmented plant populations (McCauley, 1994) • Builds and maintains seed banks(Peroni and Armstrong, 2001) • Researchers need effective way to break seed dormancy • GA3 – naturally occurring plant hormone - promotes intake of cell wall hydrolases and weakens thick cell layers of endosperm - allows radicle to emerge from the seed coat (Leubner, 2003) • Previous study indicates increasing GA3 concentration increases mean fraction germination (Bio 341, Spring 2002, unpublished data) Methods • 1000 seeds obtained from 2002 and 2003 seed crops from same population • Groups of 50 seeds randomly assigned to treatment in Petri dish - 5 replicates of each treatment (Table 1) • Petri dish – 60 mm with filter paper saturated with appropriate treatment (Fig 1) • Placed in re-sealable plastic bags in growth chamber - 14 hr light, 10 hr dark at 22°C • Censused daily for 14 d - germinated seeds removed (1 mm of radicle extended) • After 14 days ungerminated seeds tested with tetrazolium for viability (Cottrell, 1947) Fig 3. Mean seeds viable. Error bars = 1se; N=5 reps/GA3 treat/year with 50 seeds per rep GA3 Increases Germination of Viable Seeds Fig 4. Mean germination of viable seeds. Error bars = 1se; N=5 reps/GA3 treat/year with 50 seeds per rep Results GA3 Increases Germination Table 1. Description of treatments. Fig 2. Mean fraction germination of all seeds. Error bars = 1se; N=5 reps/GA3 treat/yr with 50 seeds per rep Fig 1. 60mm Petri dish with 50 seeds on saturated filter paper

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