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Microbiology Proficiency Tests and Methods in Environmental Analysis

Explore the essential proficiency tests, chain of custody procedures, and microbiology methods for water and wastewater analysis. Learn about membrane filtration, coliform testing, and calculating results in environmental microbiology.

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Microbiology Proficiency Tests and Methods in Environmental Analysis

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  1. Microbiology Overview KWWOA April 09, 2014 Department for Environmental ProtectionEnergy & Environment Cabinet

  2. Topics

  3. Topics • Proficiency Tests • Chain of Custody • Methods • Membrane Filtration

  4. Proficiency Tests

  5. Proficiency Tests • Drinking Water: WS Study SW sample for MPN • Wastewater: DMR-QA WP Study • KY Approved PT Providers List

  6. Proficiency Tests

  7. Proficiency Tests

  8. Proficiency Tests

  9. Proficiency Tests Revised reports will not be accepted. Photo: peoplesdaily.com

  10. Chain of Custody

  11. Chain of Custody • Client name & address • Sampler name • Sample location • Collection date & time • Sample type (grab/composite) • Matrix (DW/WW) • Field sample ID • Laboratory sample ID • Container type, & size/volume • Number of containers • Preservative (ice, etc.) • Field data (pH, TRC, etc.) • Requested analysis • Reason for rejection or • special remarks • Signature, date & time for • each transaction • (relinquishing & receiving) • Sample temperature upon arrival at lab

  12. BARF is NOT a COC

  13. Methods

  14. Microbiology Methods

  15. Colilert-18 P/A Colilert-18pre-warming

  16. Colilert-18 MPN

  17. Membrane Filtration

  18. Membrane Filtration Dilutions: Select appropriate volumes Fecal Coliform or E. coli colonies: 20-60* Total colonies: 200 *Use range as specified in particular method. Photo: www.pall.com

  19. Membrane Filtration Blanks: • Beginning of series • End of series • RECORD results of blanks

  20. Membrane Filtration Calculating Results: • Use dilution that is in range to determine result. • If multipleplateswithin range, average those plate counts. • If zero colonies on all plates, calculate as if 1 colony on filter from largest volume processed. Include remark “<“ in comments. Photos: www.avocetlab.com

  21. Membrane Filtration Calculating Results: • If all plates are above range, calculate using filter from smallest volume processed. Include remark “Estimated“ in comments. • WW TNTC = 60,000

  22. Contact Information Tiffany Nolan Kentucky Department for Environmental Protection Division of Water 200 Fair Oaks Lane; 4th Floor Frankfort, KY 40601 Phone: (502) 564-3410 Email: Tiffany.Nolan@ky.gov

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