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Tristan Ursell Group Meeting 10-22-2010

Tristan Ursell Group Meeting 10-22-2010. One or both of the following are true: - PG has a natural curvature that dictates cell width - MreB in vivo has a natural curvature that, via PG construction, dictates cell width . Project #1 Regrowth of the Cell Wall.

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Tristan Ursell Group Meeting 10-22-2010

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  1. Tristan UrsellGroup Meeting 10-22-2010

  2. One or both of the following are true: - PG has a natural curvature that dictates cell width - MreBin vivo has a natural curvature that, via PG construction, dictates cell width Project #1Regrowth of the Cell Wall

  3. Grow cells in penicillin or vancomycin and treat with lysozyme. Regrowth of the Cell Wall Errington Nature 2009

  4. Regrowth of the Cell Wall Errington Nature 2009

  5. Regrowth of the Cell Wall Berndl EPL 1990 Wurtis PRE 1997 Seifert PRA 1991

  6. Regrowth of the Cell Wall What proteins cause it to curve? How is the binding symmetry broken? Tseng J BiolChem 2005

  7. In what morphologies does the cell wall regrow in L-forms? (Phase, cytosolic GFP, FM 464 membrane labeling) Where is the DNA in ‘dividing’ L-forms? (DAPI labeling) Is mreB necessary for cell wall regrowth? (A2 depolymerization) Where is mreB in L-form bacteria? (mreB-GFP) How do L-forms propagate? (murE inducible strain?) Regrowth of the Cell Wall

  8. Project #2Membrane-Mediated Protein Interactions

  9. Membrane-Mediated Protein Interactions

  10. Membrane-Mediated Protein Interactions

  11. Membrane-Mediated Protein Interactions

  12. Membrane-Mediated Protein Interactions

  13. Membrane-Mediated Protein Interactions

  14. Membrane-Mediated Protein Interactions How strong is orientational ordering? To what degree can shape encode specificity? What are the kinetics of formation of different complexes? What are the different phases that exist?

  15. Membrane-Mediated Protein Interactions

  16. Membrane-Mediated Protein Interactions Mason PRL 2009

  17. Membrane-Mediated Protein Interactions Rothemund Nature 2006

  18. Membrane-Mediated Protein Interactions

  19. Membrane-Mediated Protein Interactions

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