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C4d Recomendations

C4d Recomendations. C4d staining should be done on all diagnostic renal transplant biopsies. Criteria for C4d Positivity. 1. Cryostat section, Monoclonal Ab, immunofluorescence. Widespread, strong, linear, circumferential, peritubular capillary staining in either cortex or medulla

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C4d Recomendations

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  1. C4d Recomendations • C4d staining should be done on all diagnostic renal transplant biopsies.

  2. Criteria for C4d Positivity • 1. Cryostat section, Monoclonal Ab, immunofluorescence. • Widespread, strong, linear, circumferential, • peritubular capillary staining in either cortex or medulla • Excluding scarred or necrotic areas • Concurrent positive control (essential, if neg being • reported) • Reports should include the % of area of positive PTC and intensity.

  3. Criteria for C4d Positivity • 2. Paraffin, Polyclonal Ab, immunohistochemistry • Same as frozen tissue, but • strong staining not required* • *The type of pretreatment influences the intensity. GBM/mesangial staining in the sample cannot be used as a positive control, but membranous GN is satisfactory.

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