Supplementary Figure 7 Hayashi et al

1. Control Treatment 4 x 44K custom oligo-DNA microarray chip (Catalog number G2514F: Agilent Technologies, Palo Alto, CA; GEO ID: GPL 7252) [G3 Scanner, AGILENT] Healthy at NIES and ITF (small exposure on the way to ITF) Exposure to Low-level Gamma Radiation at ITF C-0h T-6h T-72h C-72h 6-10 seedlings each = Pooled 3rd Leaves C-0h T-72h C-0h T-6h Leaf NIES NIES ITF – Iitate Farm, Iitate Village, Fukushima NIES – National Institute for Environmental Studies C-0h T-72h C-0h T-6h Differentially Expressed Genes - ITF Cy3 × × Chip 1 Dye-Swap Cy5 Differentially Expressed Genes – Control (0 h – early morning changes) (72 h – growth effects at NIES) C-0h NIES C-0h C-0h NIES C-72h NIES Cy3 × × Chip 2 Dye-Swap C-0h C-0h NIES C-72h NIES C-0h NIES Cy5 Supplementary Figure 7. DNA microarray chip/slide design and placement of control and treatment sample sets using the dye-swap approach. Two 4x44K chips were used in this study. Supplementary Figure 7 Hayashi et al