A B C Cell Density (Arbitrary Units) D Cell Density (Arbitrary Units) Gentile et al., Supplementary Figure 6 Validation of Arhgap12 localization and actions in different cellular models. (A) localization of Arhgap12-GFP in MLP-29 and HeLa cells, as indicated. (B) Scattering of control and Arhgap12 shRNA-transduced DU145 human prostate carcinoma cells in the absence or presence of HGF for three hours, as indicated. (C) Adhesion of control and Arhgap12-GFP-transduced MLP-29 cells to fibronectin, in the absence or presence of HGF, as indicated. (D) Transwell migration of control and Arhgap12-GFP-transduced MCF10A human mammary epithelial cells, in the absence or presence of HGF, as indicated. Panels C and D show means and standard deviations of three independent experiments.