1 / 1

BiFC Interactions in Tobacco Leaf Cells

Visualization of Protein-Protein Interactions in Tobacco Leaf Epidermal Cells using BiFC technique, showing dimerization on ER and PM, co-localization to ER and PM, and enhanced YFP fluorescence.

pandora-farmer
Télécharger la présentation

BiFC Interactions in Tobacco Leaf Cells

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. A1 A2 PI-PLC1-CYFP+PI-PLC1-NYFP PI-PLC1-CYFP +PI-PLC1-NYFP B2 B1 B3 PI-PLC1+Gα-Q223L ER-mCherry Merge C1 C2 C3 PI-PLC1+Gα-Q223L PM-mCherry Merge D1 C2 C1 PI-PLC1-CYFP +Clo3-NYFP Figure S8. BiFC interactions in tobacco leaf epidermal cells. A: PI-PLC1-CYFP and PI-PLC1 -NYFP dimerization on ER and PM; B:co-localization of GA3-Q223L-CYFP and PI-PLC1-NYFP interaction to ER; C:co-localization of GA3-Q223L-CYFP and PI-PLC1-NYFP to PM; Twenty cells of GA3-Q223L-CYFP and PI-PLC1-NYFP BiFC interaction have higher YFP flourecent compered to GA3-CYFP and PI-PLC1-NYFP BiFC interaction; D:the localization of the interaction between the two PI-PLC1-CYFP and Clo3-NYFP apparent by ER network structure seen in the upper focal planes of the cell. Scale bar = 24 µM

More Related