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BMEN 423/621 Laser Microscopy

BMEN 423/621 Laser Microscopy. Alvin T. Yeh ayeh@tamu.edu http://biomed.tamu.edu/tml. K. Konig J. Microsc. 200: 83-104 (2000). Microscopic Imaging. Light- vs. Dark-field imaging Stained vs. unlabeled Vital vs. fixed. Increasing use of Laser Microscopy.

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BMEN 423/621 Laser Microscopy

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  1. BMEN 423/621Laser Microscopy Alvin T. Yeh ayeh@tamu.edu http://biomed.tamu.edu/tml

  2. K. Konig J. Microsc. 200: 83-104 (2000).

  3. Microscopic Imaging Light- vs. Dark-field imaging Stained vs. unlabeled Vital vs. fixed

  4. Increasing use of Laser Microscopy W.R. Zipfel, R.M. Williams, W.W. Webb Nat. Biotechnol. 21(11): 1369-1377 (2003). • commercialization of laser microscopy systems • increased technical savvy of biologists • benefit to biomedical studies

  5. Technically Demanding W.R. Zipfel, R.M. Williams, W.W. Webb Nat. Biotechnol. 21(11): 1369-1377 (2003).

  6. Laser-Tissue Interaction K. Konig J. Microsc. 200: 83-104 (2000). Does this schematic capture photophysics correctly?

  7. Information Content

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