1 / 17

S 2007 BIOC 3406

This document delves into key biochemical pathways, illustrating the complexity of ATP cleavage at three crucial points. It covers the conversion of ATP to ADP and AMP, exploring the role of inorganic phosphate (PPi) and the adenylylation process in DNA and RNA synthesis. The significance of ion transport, particularly Na+/K+ ATPase behavior in establishing membrane potentials, is discussed alongside biochemical equations focused on half-reactions. Additionally, it emphasizes redox processes, reduction potentials, and the role of electron carriers like NADH and FAD in metabolic reactions.

pooky
Télécharger la présentation

S 2007 BIOC 3406

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. S 2007 BIOC 3406 01-23-07

  2. Three cleavage points for ATP ROH + ATP  • ADP leaving group, ROPO32- + ADP • AMP leaving group, ROP2O63- + AMP • P2O74- (PPi) leaving group, adenosine-R ester product (R has been adenylylated)

  3. Adenylylation in the synthesis of DNA, RNA • NTP • ROH is polymer chain 3’ terminal ribose

  4. K+ Na+ Active Xport • Na+/K+ ATPase • Moves Na+ across cell membranes • Na+ out and K+ in • Establishes electrical, chemical potential Na+ Higher potential Lower potential K+

  5. Inorganic polyphosphate • 0.2 M in yeast vacuoles • Transport of H+ in plants • Prebiotic energy system

  6. Biochemical Equations • More focused than ordinary • Often not balanced, either in charge OR atoms • Emphasizes substrate (often ignores enzyme) • Assumes pH 7 and 1mM Mg2+

  7. Half-Reactions • Red  Red+ + e- • Ox + e-  Ox- • Total: Red + Ox  Red+ + Ox-

  8. Dehydrogenation • Transfer of H2 is a redox • Hydrogenation of a double bond • Hydrogenation across conjugated carbon chain

  9. Reduction Potentials e- flow ion flow Red, Red+ Ox, Ox- Pt electrodes salt bridge anode cathode

  10. Nernst E = E0 +(RT/nF)ln[(eln acceptor)/(electron donor) G ~ E G = -nFE

  11. Electron carriers • NADH • FAD

  12. Problem 18

More Related