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Immunohistochemistry Immunofluorescence Introduction to research

Immunohistochemistry Immunofluorescence Introduction to research

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Immunohistochemistry Immunofluorescence Introduction to research

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  1. ImmunohistochemistryImmunofluorescenceIntroduction to research

  2. Why use it? Look for specific protein expression within tissues Study development, diseases, and evolution

  3. Western Blot • Same technology is used for western blotting

  4. Protein expression: p63

  5. How it works

  6. Different methods – pro’s and con’s

  7. Double-Labeling Co-localization of proteins Compartmentalized expression Paracrine expression

  8. Immunofluorescence • Need special microscope for study