1. ImmunohistochemistryImmunofluorescenceIntroduction to research

2. Why use it? Look for specific protein expression within tissues Study development, diseases, and evolution

3. Western Blot • Same technology is used for western blotting

4. Protein expression: p63

5. How it works

6. Different methods – pro’s and con’s

7. Double-Labeling Co-localization of proteins Compartmentalized expression Paracrine expression

8. Immunofluorescence • Need special microscope for study