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MIT’s Eau d’e coli iGEM 2006

MIT’s Eau d’e coli iGEM 2006. By: Robert Ovadia. 1. Background System Design Results What I Liked and Disliked. Background. What makes a scent? An enzyme that converts something that DOESN’T smell, into something that DOES smell. JMT jasmine. CCMT cinnamon. BAMT fresh / floral.

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MIT’s Eau d’e coli iGEM 2006

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  1. MIT’s Eau d’e coli iGEM 2006 By: Robert Ovadia 1

  2. Background System Design Results What I Liked and Disliked Background What makes a scent? An enzyme that converts something that DOESN’T smell, into something that DOES smell. JMT jasmine CCMT cinnamon BAMT fresh / floral BSMT wintergreen ATF1 banana 2

  3. Background System Design Results What I Liked and Disliked Background BSMT wintergreen ATF1 banana 3

  4. Background System Design • WGD • BGD • SAGD • IAGD • pOsmY + Inverter Results What I Liked and Disliked System Design 4

  5. Background System Design • WGD • BGD • SAGD • IAGD • pOsmY + Inverter Results What I Liked and Disliked System Design Simple System 5

  6. Background System Design • WGD • BGD • SAGD • IAGD • pOsmY + Inverter Results What I Liked and Disliked System Design Complex System 6

  7. Background System Design • WGD • BGD • SAGD • IAGD • pOsmY + Inverter Results What I Liked and Disliked System Design More Complex System 7

  8. Background System Design • WGD • BGD • SAGD • IAGD • pOsmY + Inverter Results What I Liked and Disliked System Design Complete System 8

  9. Background System Design • WGD • BGD • SAGD • IAGD • pOsmY + Inverter Results What I Liked and Disliked System DesignWintergreen Generating Device (WGD) 9

  10. Background System Design • WGD • BGD • SAGD • IAGD • pOsmY + Inverter Results What I Liked and Disliked System DesignBanana Generating Device (BGD) 10

  11. Background System Design • WGD • BGD • SAGD • IAGD • pOsmY + Inverter Results What I Liked and Disliked System DesignSalicylic Acid Generating Device (SAGD) 11

  12. Background System Design • WGD • BGD • SAGD • IAGD • pOsmY + Inverter Results What I Liked and Disliked System DesignIsoamyl Alcohol Generating Device (IAGD) 12

  13. Background System Design • WGD • BGD • SAGD • IAGD • pOsmY + Inverter Results What I Liked and Disliked System DesignOsmY + Inverter • osmY: active in stationary phase & under high osmotic pressure conditions osmY osmY + inverter 13

  14. E. coli + precursor (SA) + WGD E. coli methyl salicylate MW = 152 no methyl salicylate [no peak at 9.1 minutes] Background System Design Results • WGD • Growth Dependent Regulation • Growth Dependent Regulation + Inverter • Indole Free Bacteria • Smell Test What I Liked and Disliked ResultsWintergreen Generating Device 14

  15. Background System Design Results • WGD • Growth Dependent Regulation • Growth Dependent Regulation + Inverter • Indole Free Bacteria • Smell Test What I Liked and Disliked ResultsGrowth Dependent Regulation 15

  16. Background System Design Results • WGD • Growth Dependent Regulation • Growth Dependent Regulation + Inverter • Indole Free Bacteria • Smell Test What I Liked and Disliked ResultsGrowth Dependent Regulation + Inverter 16

  17. wild type E. coli chassis: tnaA5-E. coli Indole Peak at 10 minutes MW = 117 No indole [no peak at 10 minutes] Background System Design Results • WGD • Growth Dependent Regulation • Growth Dependent Regulation + Inverter • Indole-Free Bacteria • Smell Test What I Liked and Disliked ResultsIndole-Free Bacteria 17

  18. Background System Design Results • WGD • Growth Dependent Regulation • Growth Dependent Regulation + Inverter • Indole-Free Bacteria • Smell Test What I Liked and Disliked ResultsSmell Test 18

  19. Background System Design Results What I Liked and Disliked • Likes What I Liked Likes • Able to change a simple experiment into a more complicated experiment • They looked at what MORE there was to do; took their experiment to the ‘next level’ EX: Simple System to Complete System • Got the audience involved, EX: Smell test • Easy to comprehend • Perfect example of a successful iGEM project 19

  20. Sources MIT’s iGEM PPT Presentation 2006.ppt (PowerPoint) MIT’s iGEM PPT Presentation 2006.mov (Movie) http://openwetware.org/wiki/IGEM:MIT/2006 20

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