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Specific Recognition of MUC20 Overexpression by anti-MUC20 Antibody

Western blotting confirmed the anti-MUC20 antibody's specificity for MUC20 in HEK293 cells, validated by immunohistochemistry in human kidney tissues. The antibody strongly stained proximal tubules, consistent with prior findings. IgG control lacked specific binding.

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Specific Recognition of MUC20 Overexpression by anti-MUC20 Antibody

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  1. A B Vector control MUC20 anti-MUC20 antibody IgG control (kDa) 250 MUC20 130 * 55 36 50 μm GAPDH HEK293 Supplementary Fig. S1. Specificity of anti-MUC20 antibody. (A) Western blotting showed that anti-MUC20 antibody specifically recognizes MUC20 overexpressed in HEK293 cells. The arrow indicates MUC20 protein. GAPDH is an internal control. (B) Immunohistochemistry of MUC20 in human kidney. The anti-MUC20 antibody strongly stained proximal, but not distal, convoluted tubules, which is consistent with the previous report (J Biol Chem. 2004;279:1968-79). The star (*) indicates the distal convoluted tubule. IgG control did not show specific binding.

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