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Vector control

A. B. Vector control. MUC20. a nti-MUC20 antibody. IgG control. ( kDa ). 250. MUC20. 130. *. 55. 36. 50 μ m. GAPDH. HEK293.

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Vector control

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  1. A B Vector control MUC20 anti-MUC20 antibody IgG control (kDa) 250 MUC20 130 * 55 36 50 μm GAPDH HEK293 Supplementary Fig. S1. Specificity of anti-MUC20 antibody. (A) Western blotting showed that anti-MUC20 antibody specifically recognizes MUC20 overexpressed in HEK293 cells. The arrow indicates MUC20 protein. GAPDH is an internal control. (B) Immunohistochemistry of MUC20 in human kidney. The anti-MUC20 antibody strongly stained proximal, but not distal, convoluted tubules, which is consistent with the previous report (J Biol Chem. 2004;279:1968-79). The star (*) indicates the distal convoluted tubule. IgG control did not show specific binding.

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