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Flow Cytometry

Flow Cytometry

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Flow Cytometry

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  1. Flow Cytometry Melanie, Jade, and Brooke

  2. Flow cytometry is a measurement of physical and chemical properties that is made while the cells pass, in single file, through the apparatus in a fluid stream. http://www.unsolvedmysteries.oregonstate.edu/flow_cytometry_06.shtml What Is This Method?

  3. Why? • Measures a cell’s diameter, volume, surface area, and granularity. • Identifies and quantifies different types of cells by their surface proteins. • Determines a cell’s internal structure, including its DNA content, enzymes, and proteins. • The process is very rapid (1000 cells/s)

  4. What Is Some Typical Data?

  5. Real Data

  6. References • Howard Shapiro. “Practical Flow Cytometry”. John Wiley and Sons Inc., New York. • http://qbab.aber.ac.uk/aberinst/mcytmain.html • http://www.unsolvedmysteries.oregonstate.edu/flow cytometry 07.shtml • “Intestinal epithelial cell-derived interleukin-7: A mechanism for the alteration of intraepithelial lymphocytes in a mouse model of total parenteral nutrition.” Hua Yang, Xiaoyi Sun, Emir Haxhija, and Daniel H. Teitelbaum. Section of Pediatric Surgery, Department of Surgery, the University of Michigan Medical School and the C. S. Mott Children’s Hospital, Ann Arbor, Michigan.2007.