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The protocol involves starch hydrolysis with amylase, adding iodine reagent to detect dextrin transformation, and reading optical density. Detailed steps and iodine solutions are provided for accurate results. Ensure safety precautions during the process.
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Lyven protocol • For each erlen: following of starch hydrolyze • Removal of 0.5 ml of starch solution • Adding 2.5 ml iodine reagent • Mixing by reversal • Read optical density at 630 nm • At the beginning starch is blue dark. Then it turns to brown when it is transformed in dextrin with amylase activity. At the end of hydrolyze the solution stay yellow Composition
Starch Dextrin Time of hydrolyze with amylase
Iodine reagent Return • Iodine concentrated solution: • 4.4 g of potassium iodure (KI) • 2.2 g of iodine (I2) • Dissolve successively in about 20 ml of distilled water, complete to 100 ml • Store this solution in dark glass maximum one month • Iodine reagent: • Dissolve 4g of potassium iodure in about 80 ml of distillated water • Add 0.4 ml of iodine concentrated solution • Complete to 100 ml with distilled water • Store maximum one day • Individual protection equipment is recommended for iodine handling
