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Characterization of Maize Chromosome Addition Lines with Unique Traits and Genomic Mapping

This study explores the unusual characteristics of maize chromosome addition lines, particularly focusing on Chromosome 1 and Chromosome 10. Notable traits include erect leaf blades, a photoperiod-neutral response, and sectoring among shoots. The integration of wheat and maize was assessed using genomic in situ hybridization techniques and high-energy gamma rays. We also investigated the mapping of specific sequences using primers designed to amplify ESTs from maize chromosomes, contributing to the understanding of genetic relationships and the potential presence of multiple alleles.

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Characterization of Maize Chromosome Addition Lines with Unique Traits and Genomic Mapping

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  1. Chromosome 1 addition line Unusual characteristics noticed in chromosome 1 OMA lines:Erect leaf blade, photoperiod neutral response, sectoring among shoots for the maize chromosome

  2. Chromosome 10 Addition Line Characteristics of chromosome 10 OMA lines: grassy type and very sterile

  3. Oat x maize addition line Genomic in situ hybridization of an addition line with fluorescein-labeled maize DNA. The pair of maize chromosomes are yellow-green, and the oat chromosomes are counterstained red with propidium iodide.

  4. 30-40 krad γ-rays Oat-corn chromosome 9 monosomic addition line Selfing Oat line without a corn chromosome Oat line with a corn chromosome piece transfer Oat line with a modified corn chromosome

  5. Mapping with OMA lines primer33C primer33A primer34A primer34C primer34A: will amplify ESTs AW066336 and AW066011. These two sequences are very similar, e-172 and 335/343 (97%) and may represent two alleles from one gene. primer34C: was designed to amplify TC77059. This sequence is more distantly related to AW066336, e-112, 269/289 (93%). The gene represented by ESTs AW066336 and AW066011 appears to lie on chromosome 5. The PCR primers, primer34C, detects the related sequence on chromosome 4.

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