'Agarose gel electrophoresis' diaporamas de présentation

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ELECTROPHORESIS Definitions Theory of Electrophoresis Electrophoretic Technique General Procedures Types of Electrophore

ELECTROPHORESIS Definitions Theory of Electrophoresis Electrophoretic Technique General Procedures Types of Electrophoresis Technical Considerations Ref: Burtis & Ashwood; Tietz Fundamentals/Textbook. 1. DEFINITIONS Electrophoresis

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Polymerase Chain Reaction (PCR2)

Polymerase Chain Reaction (PCR2) fourth lecture Zoology department 2007 Dr.Maha H. Daghestani PCR Definition of PCR Requirements for PCR PCR Process A. Denaturing Stage B. Annealing Stage C. Extending Stage Polymerase Chain Reaction (PCR

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Ch 9 Frontiers of Biotechnology

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Discover the Microbes Within

Discover the Microbes Within. How many species have Wolbachia?. Agarose Gel Electrophoresis. Gel electrophoresis is a widely used technique for the analysis of nucleic acids and proteins . Agarose gel electrophoresis is routinely used for the preparation and analysis of DNA.

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Sixth Framework Programme Priority 1 Life Sciences, Genomics and Biotechnology for Health

PolExGene. Sixth Framework Programme Priority 1 Life Sciences, Genomics and Biotechnology for Health. Gene delivery related research at UGent. PolExGene meeting Tuebingen, 20/12/2006. P. Dubruel , V. Vermeersch and E. Schacht. Introduction. Current status:

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Gel Electrophoresis

Gel Electrophoresis. Prepered by:- Rana Al-Turki. Agarose Gel Electrophoresis. Gel electrophoresis is a widely used technique for the analysis of nucleic acids and proteins . Agarose gel electrophoresis is routinely used for the preparation and analysis of DNA.

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Separation: Agarose gel electrophoresis-I

Section G-II Gene Manipulation G3 Electrophoresis G4-1 Ligation G4-2 Transformation and G4-3 Analysis of recombinants. -. +. DNA sample. buffer solution. Migration of DNA. Agarose gel. Separation: Agarose gel electrophoresis-I.

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DNA Structure and Analysis

DNA Structure and Analysis. Chapter 4: Background. Molecular Biology. Three main disciplines of biotechnology Biochemistry Main focus on proteins and their function Genetics Main focus on genes and their function Molecular Biology Main focus on genes and the proteins they make.

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RNA Electrophoresis

RNA Electrophoresis. Broad and Long Term Objective. To characterize the expression of ribulose 1-5 bisphosphate carboxylase oxygenase and chlorophyll AB binding gene in Lycopersicon esculentum (Tomato) leaves subjected to 24, 48, and 72 hours in the dark. Research Plan.

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Electrophoresis of DNA

Electrophoresis of DNA. What is it used for? How does it work? How do we do it? What are the results?. What is electrophoresis?. Separation of charged molecules DNA has net negative charge. -. +. What is Agarose?. A polysaccharide polymer Prepared from seaweed Highly purified

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DNA Structure and Analysis

DNA Structure and Analysis. Activity 4.3: Dye Electrophoresis . Activity 4.3: Dye Electrophoresis. Research questions: What charge do dyes have? Are samples composed of multiple dyes? Objectives: Load and run dyes on an agarose gel Determine the charge of each electrophoresed dye

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Gel Electrophoresis

Gel Electrophoresis. Measurement of DNA Concentration. Agarose Gel Electrophoresis ( Bases for DNA separation) Agarose melted in a buffer and poured into a horizontal tray When solidified, the gel contains small holes or pores through which the DNA fragments will travel

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1% Agarose Gel DNA Electrophoresis

1% Agarose Gel DNA Electrophoresis. Running time : 52 minutes Distance measured from the well to the top of the second band: 4,5 cm Running voltage: 100 Volt Solidification time: 14 minutes Composition: 250 mikroL buffer TAE + 2,5 gram Agarose + 20 mikroL Ethidium Bromide. 100 Kb. 1 Kb.

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Restriction Mapping of a Plasmid

Restriction Mapping of a Plasmid. Once a plasmid or any DNA sample is cut with restriction enzymes, we havew to have a way to visualize the pieces: Agarose Gel Electrophoresis. http://www.wiziq.com/tutorial/22367-Gel-Electrophoresis-Animation Three relevant facts about gel electrophoresis:

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Nene Aissatou Diallo

Nene Aissatou Diallo. Present my PI Talk about the electrophoresis of DNA Then the Gel red. From M exico Did his education in california Cell Biology, Genetics and Molecular Biology of Plants He always has this question How do things grow? Relation between Bacterium and plants.

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Agarose Gel Electrophoresis

Agarose Gel Electrophoresis . O. O. P. O. Base. O. O. CH 2. Sugar. Negative charges. O. O. O. P. Base. O. O. CH 2. Sugar. OH. Deoxyribonucleic Acid (DNA). Moves towards a positive charge . Agarose Matrix.

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At18S-Adapter.R1

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Novel Functions of PXR: A Bioinformatic Approach

Novel Functions of PXR: A Bioinformatic Approach. Callum Allison , Hannah Sian-McGuiness, Dr Ian Bailey Contact: i.bailey@westminster.ac.uk. Introduction:

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a. b.

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