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1% Agarose Gel DNA Electrophoresis

1% Agarose Gel DNA Electrophoresis. Running time : 52 minutes Distance measured from the well to the top of the second band: 4,5 cm Running voltage: 100 Volt Solidification time: 14 minutes Composition: 250 mikroL buffer TAE + 2,5 gram Agarose + 20 mikroL Ethidium Bromide. 100 Kb. 1 Kb.

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1% Agarose Gel DNA Electrophoresis

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  1. 1% Agarose Gel DNA Electrophoresis Running time : 52 minutes Distance measured from the well to the top of the second band: 4,5 cm Running voltage: 100 Volt Solidification time: 14 minutes Composition: 250 mikroL buffer TAE + 2,5 gram Agarose + 20 mikroL Ethidium Bromide 100 Kb 1 Kb Scale in Cm Second Band

  2. 2% Agarose Gel DNA Electrophoresis Running time : 52 minutes Distance measured from the well to the top of the second band: 3.4 cm Total Running time: 62 minutes Total distance measured: 4.5 cm Running voltage: 100 Volt Solidification time: 21 minutes Composition: 500 mL buffer TAE + 10 gram Agarose + 40 mikroL Ethidium Bromide 100 Kb 1 Kb Scale in Cm Second Band

  3. 3% Agarose Gel DNA Electrophoresis Running time : 52 minutes Distance measured from the well to the top of the second band: 2.9 cm Total Running time: 68 minutes Total distance measured: 4.5 cm Running voltage: 100 Volt Solidification time: 17 minutes Composition: 250 mikroL buffer TAE + 7.5 gram Agarose + 40 mikroL Ethidium Bromide 100 Kb 1 Kb Scale in Cm Second Band

  4. Overall comparison 1% 2% 3% Cross sections of 3 different concentration of DNA Agarose Gel are illuminated under UV light

  5. Overall Comparison 1% 3% 2% Increasing the concentration of Agarose gel decreases the electrpphoretic mobility of DNA Larger Fragments are better resolved in the low agarose concentration while higher concentration allow resolution of smaller DNAs 1 kb 1 kb 100 bp 100 bp 100 bp 1 kb

  6. Comparison5 mikroL of 1 Kb DNA Ladder in 1%,2% and 3% Agarose Gel Electrophoresis 1% 2% 3% Wells level 3,0 kb 2,0 kb 3,0 kb 2,0 kb

  7. Comparison5 mikroL of 100 bp DNA Ladder in 1%,2% and 3% Agarose Gel Electrophoresis 1% 2% 3% Wells level 1000 bp 500 bp 1000 bp 500 bp

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