'Intron' diaporamas de présentation

Gene Expression: Transcription and Translation, Chapters 5 and 6

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Application of Bioinformatics, Proteomics, and Genomics

Application of Bioinformatics, Proteomics, and Genomics. Introns, Spcicing, and Alternative splicing. Out of the coding business. STATISTICS. 92% of mammalian genes have exon/intron structures while only 8% of genes are intron-free. The average segmented gene of these species

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Polymerase Chain Reaction

Polymerase Chain Reaction. PCR. (PCR). P C R. PCR produces billions of copies of a specific piece of DNA from trace amounts of starting material. (i.e. blood, skin cells, bone) Allows scientists to isolate pure quantities of specific DNA sequences

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Viya Fedoseyeva Russian Academy of Sciences, Russia Institute of Molecular Genetics

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Explicit length distribution models in Iscan

Explicit length distribution models in Iscan. Lab meeting 8/20/2003 By Chaochun Wei. Length distribution models in Iscan. Explicit length distribution models: Exon and intron. Effect of the intron length limit. Effect of the smoothlization of the length distribution models.

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Identifying exons for PCR primer design in Plasmodium falciparum

gene. exon. exon. exon. intron. intron. transcription. mRNA prior to processing. mRNA processing. Processed mRNA. translation. protein. Identifying exons for PCR primer design in Plasmodium falciparum.

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Gene Expression

Gene Expression. CHS superfamily genes from the moss P. patens. Gene Gene location Seq. identity Remarks Scaffold:base to PpCHS (%) PpCHS1a 500:75157 99.2 No intron. Differ at 3 a.a. from PpCHS PpCHS01 22:12268 99.5 Differ at 2 a.a. and 3?-UTR from PpCHS1a

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UVM11

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A C K L

A:. In-vivo minigene In-vitro RNA template. Exon 11 skipping: HepG2 HeLa. Intron. GA INH. pIRA pIRC pIRK pIRL. A:. 255 nt. 11. 10. 14% 13%. 188 nt. 0% 0%. C:. 151 nt. 51% 68%. K:. 84 nt. 22% 17%. L:. B:.

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(a) bp difference between mouse and human / bp of the larger corresponding intron.

(a) bp difference between mouse and human / bp of the larger corresponding intron.

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259

TGA. ATG. Intron (kb). 2.4. 12.1. 71.9. 25.8. 16.5. 36.7. 21.8. 16.3. 23.4. 5.5. H . sapiens (522 aa ). Exon (bp). 259. 90. 149. 173. 177. 110. 108. 141. 138. 98. 126. Intron phase. 1. 1. 0. 2. 2. 1. 1. 1. 1. 0. ATG. TGA. Intron (kb). 2. 8. 9.0. 53.3.

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10h

(a). Tgt1 (9,446bp). Xba I. Xba I. Eco RI. Eco RI. PBS. PPT. LTR. LTR. 2180bp. 2180bp. AGCAG. AGCAG. ORF1 (488aa). ORF2 (1152aa). intron. f3?5?h2 Tgt1. exon1. exon2. (b). 1. 2. 1. 2. (kbp). 10-. 6-. 4-. 3-. 2-. 1-. 1h. 10h. Exposure time. Fig. S5.

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