'Lacz gene' diaporamas de présentation

Chapter 20

Chapter 20. Biotechnology. Overview: The DNA Toolbox. Sequencing of the human genome was completed by 2007( 3 million base pairs) DNA sequencing has depended on advances in technology, starting with making recombinant DNA

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Biotechnology and Genetic Engineering

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Mobile & Transposa ble Elements

J u m pi n g G e n e s. Mobile & Transposa ble Elements. Ethnobotany. mosaic kernels . 1938: Marcus Rhoades reported odd phenotypic ratios in corn. Self pollination of a pigmented corn kernel yielded: 12 : 3 : 1 pigmented : d o t t e d : colorless. A hypothesis:

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Chapter 20

Chapter 20. Biotechnology. Overview: The DNA Toolbox. Sequencing of the human genome was completed by 2007 DNA sequencing has depended on advances in technology, starting with making recombinant DNA

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DNA

DNA. How is the expression of genes controlled in prokaryotes? What are some ways the expression of genes are controlled in eukaryotes? What are histones?. DNA Technology. Meet Dolly. Biotechnology. The manipulation of organisms or use of living things as technology

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Chapter 20

Chapter 20. Biotechnology. Why biotechnology?. Diagnosis of disease Gene therapy Pharmaceutical development Forensic applications Environmental remediation Agricultural applications. Overview: The DNA Toolbox. Sequencing of the human genome was completed by 2007

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Chapter 20

Chapter 20. Biotechnology. Overview: The DNA Toolbox. Sequencing of the human genome was completed by 2007 DNA sequencing has depended on advances in technology, starting with making recombinant DNA

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Chapter 20

Chapter 20. DNA Technology and Genomics. Overview: Understanding and Manipulating Genomes One of the greatest achievements of modern science Has been the sequencing of the human genome, which was largely completed by 2003 DNA sequencing accomplishments

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Biotechnology (Ch.20)

Biotechnology (Ch.20). A Brave New World. human genome 3.2 billion bases.

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Methods in Gene Cloning

17.3. Methods in Gene Cloning. Objectives. describe the steps in gene cloning by using plasmid as the vector. The steps for cloning:. 1) Isolation. 2) Splicing. 3) Insertion. 4) Transformation. 5) Screening. Steps in Gene Cloning. 1. Isolation of DNA (gene).

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DNA Technology and Genomics

Understanding and Manipulating Genomes One of the greatest achievements of modern science Has been the sequencing of the human genome, which was largely completed by 2003 DNA sequencing accomplishments

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What do you notice about these phrases?

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What do you notice about these phrases?

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Bacterium

Bacterium. Cell containing gene of interest. Gene inserted into plasmid. 1. 2. 4. 3. Gene of interest. Plasmid. Bacterial chromosome. DNA of chromosome. Recombinant DNA (plasmid). Plasmid put into bacterial cell. Recombinate bacterium.

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Bacterial Transformation

Bacterial Transformation. Lab 6A. Our Goals. Insert foreign gene into plasmid Insert recombinant plasmid into bacteria Grow transformed bacteria. Questions We Need Answered. Did the bacteria take up the plasmid? If so, did the gene of interest get inserted into the plasmid?.

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Chapter 20

Chapter 20. DNA Technology and Genomics. Figure 20.1 DNA microarray that reveals expression levels of 2,400 human genes (enlarged photo). Bacterium. Cell containing gene of interest. Gene inserted into plasmid. 4. 2. 3. 1. Gene of interest. Plasmid. Bacterial chromosome.

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iGEM UCLA

iGEM UCLA. What is Synthetic Biology?. A) the design and construction of new biological parts, devices, and systems, and B) the re-design of existing, natural biological systems for useful purposes. Useful?. Bioremediation Energy Sources Medical Systems Biology. Bioremediation.

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A Little More Advanced Biotechnology Tools

A Little More Advanced Biotechnology Tools. Better Plasmids. Engineered plasmids. Building custom plasmids restriction enzyme sites antibiotic resistance genes as a selectable marker. Eco RI. Bam HI. Hin dIII. restriction sites. Selectable marker antibiotic resistance gene on plasmid

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A Little More Advanced Biotechnology Tools

A Little More Advanced Biotechnology Tools. Better Plasmids. Engineered plasmids. Building custom plasmids restriction enzyme sites antibiotic resistance genes as a ________________. Eco RI. Bam HI. Hin dIII. restriction sites. Selectable marker antibiotic resistance gene on plasmid

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