'Southern blotting' diaporamas de présentation

Review from last time

985 views • 88 slides

UNIT 4

UNIT 4. Techniques used in Molecular Biology. Objectives. On completion of this unit students will be able to: Outline the steps in Polymerase Chain Reaction Analyse DNA agarose gel electrophoretograms Describe DNA hybridization and its application in probe synthesis

925 views • 79 slides

Mechanisms of Immune Evasion by Tumors

Mechanisms of Immune Evasion by Tumors. W.H. Chambers, Ph.D. Associate Director for Basic Research University of Pittsburgh Cancer Institute Associate Professor of Pathology University of Pittsburgh School of Medicine. DCs migrate to Secondary Lymphoid Organs To Stimulate Immunity.

799 views • 45 slides

Chapter 13

Chapter 13. DNA: The Indispensable Forensic Science Tool. Objectives:. Describe and demonstrate DNA collection, preservation, and testing, using gel electrophoresis. Use Product Rule to quantify and interpret results. Father of DNA fingerprinting. Alec Jeffreys 1985

625 views • 34 slides

Restriction Enzyme Digestion & Southern Blotting of DNA

Restriction Enzyme Digestion & Southern Blotting of DNA. Experiment Goals. Digestion of DNA by restriction enzyme Analyze digested DNA by electrophoresis Transfer digested DNA to nitrocellulose filters (Southern blotting). Restriction Enzymes.

907 views • 34 slides

Forensic Biology by Richard Li

Chapter 17: Variable-Number Tandem Repeats Profiling. Forensic Biology by Richard Li. Basics. Human genome is abundant in tandem repeats Minisatellites- 1980 Also called Variable-Number Tandem Repeats (VNTRs) Repeat unit > 10 bp (definition) Often dozens to hundreds of bp per repeat

629 views • 24 slides

GEL ELECTROPHORESIS

GEL ELECTROPHORESIS. Gel Electrophoresis. Separation of molecules by size, charge, or shape PAGE ( P oly A crylamide E el E lectrophoresis ) Used to separate proteins Proteins are kept the same shape by detergent (SDS) Agarose Used to separate DNA fragments DNA can be recovered from gel.

700 views • 20 slides

A Multicentre Technology Assessment of the Abbott Fragile X Assay

404 views • 17 slides

What I Learned about Southern Blotting By: Matthew Garma (LCC) A.B.E. Workshop 2006

405 views • 16 slides

Restriction Fragment Length Polymorphism ( RFLP )

1.1k views • 14 slides

341 views • 13 slides

DNA Probing

DNA Probing. By: Jennifer Oxford, Jacob Carter, Elemuel Coleman. Table of Contents. What is a DNA Probe? DNA Probe Simplified Process of Searching for DNA/RNA Sequence Important Processes of Hybridization Southern and Northern Blotting Blotting Method Blotting Methods Recap

844 views • 11 slides

DNA Probing

DNA Probing. By: Jennifer Oxford, Jacob Carter, Elemuel Coleman. Table of Contents. What is a DNA Probe? DNA Probe Simplified Process of Searching for DNA/RNA Sequence Important Processes of Hybridization Southern and Northern Blotting Blotting Method Blotting Methods Recap

360 views • 11 slides

NORTHERN BLOTTING

NORTHERN BLOTTING. Presented By : *M. UZAIR NIAZ *WAQAR ALI. Contents. What is Blotting? Northern Blotting History and introduction Principles of Northern Blotting Procedure Advantages and disadvantages Question answers. Blotting.

1.23k views • 10 slides

Biotech

Biotech. Southern Blotting. Through a series of steps, DNA that has been separated by electrophoresis is applied to a membrane of nylon or nitrocellulose . PCR.

221 views • 8 slides

TOOLS OF GENETIC ENGINEERING

TOOLS OF GENETIC ENGINEERING. By: Russel Jean Gallo. Recombinant DNA Technology and Procedures. Molecular cloning permits the replication of a specific DNA sequence in a living microorganism.

480 views • 8 slides

Rainbow Animals

Rainbow Animals. Rainbow Animals. Ashton Somers. Ashton Somers. Ever dreamt of having a rainbow pet?. Rainbow pets are extremely rare genetically modified organisms which first originated in Australia.

388 views • 8 slides

191 views • 8 slides

RECOMBINANT DNA AND BIOTECHNOLOGY.

RECOMBINANT DNA AND BIOTECHNOLOGY. Overview of recombinant DNA proceedures. Selection. Mutation. Restriction Enzymes. Vectors. PCR. TECHNIQUES OF GENETIC ENGINEERING. Transformation. Electroporation. Protoplast Fusion. Microinjection. Therapeutic applications of genetic engineering.

216 views • 6 slides

Restriction Fragments and Mapping

Restriction Fragments and Mapping. Restriction Fragment Analysis System used to compare the genes and DNA sequences between individuals in a population. can be used to identify heterozygous carriers of mutant alleles Uses restriction enzymes to digest (break apart) DNA into shorter segments.

669 views • 5 slides