'Sybr green' diaporamas de présentation

Real-Time PCR M.Tevfik DORAK, MD PhD updated on March 1, 2010 ( www )

Real-Time PCR M.Tevfik DORAK, MD PhD updated on March 1, 2010 ( www ). Cockerill FR III. Arch Pathol Lab Med. 2003;127:1112 (www). What is Wrong with Agarose Gels?. * Poor precision * Low sensitivity * Short dynamic range < 2 logs * Low resolution * Non-automated

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1.2k views • 88 slides

Real-Time PCR

1.71k views • 82 slides

Real-Time PCR

David A. Palmer, Ph.D. Technical Support, Bio-Rad Laboratories Adjunct Professor, Contra Costa College. Real-Time PCR. Objectives. This presentation will cover the following topics: What is real-time PCR used for? How does real-time PCR work? What instruments are used?

1.32k views • 74 slides

Dr. Chaim Wachtel April 11, 2013

Dr. Chaim Wachtel April 11, 2013. Introduction to Real-Time PCR. Real-Time PCR. What is it? How does it work How do you properly perform an experiment Analysis.

974 views • 69 slides

Dr. Chaim Wachtel June 4, 2015

Dr. Chaim Wachtel June 4, 2015. Introduction to Real-Time PCR. Summary of Last Week. Microarrays. HTS (High Throughput Sequencing). Sequence entire DNA/RNA content of sample No need for prior knowledge Artifacts- in general there is at least one amplification step

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PHYSICAL MAPPING AND POSITIONAL CLONING

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Real Time PCR = Quantitative PCR

Real Time PCR = Quantitative PCR. on genomic DNA= measure the copy number of a genomic target sequence. on cDNA = compare transcription level of a target gene between different samples (RT-PCR). Goal of Q-PCR:

784 views • 38 slides

More Molecular Genetic Technologies (Chapters 8, 9, 10) Polymerase Chain Reaction (PCR) Standard PCR method Real-time qu

More Molecular Genetic Technologies (Chapters 8, 9, 10) Polymerase Chain Reaction (PCR) Standard PCR method Real-time quantitative PCR DNA sequencing Manual dideoxy/automated fluorescent dye Pyrosequencing DNA Fingerprinting (DNA typing/profiling)

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HPV

HPV. Carcinoma of the Cervix. Many risk factors for development of cervical cancer. no routinely used positive predictive biological markers, which identify women at risk of developing high-grade lesions and ultimately invasive cancer. Human Papillomavirus (HPV).

338 views • 23 slides

Quantification of RNA by real-time PCR

Quantification of RNA by real-time PCR. Vilborg Matre. Overview. Real-time PCR. Gene expression profile - to characterize how a cell/animal responds to a stimulus. Replaces Northerns. Conventional PCR. One cycle. Denaturation. = a cyclic process leading to exponential

328 views • 19 slides

Quantification of RNA by real-time PCR

Quantification of RNA by real-time PCR. Vilborg Matre. Overview. Real-time PCR. Gene expression profile - to characterize how a cell/animal responds to a stimulus. Replaces Northerns. Conventional PCR. One cycle. Denaturation. = a cyclic process leading to exponential

573 views • 19 slides

Amplification Plots: Rab7

Amplification Plots: Rab7. SYBR Green, 03-04-2009, 441 project 1st run.mxp. Dissociation Curve: Rab7. SYBR Green, 03-04-2009, 441 project 1st run.mxp. Amplification Plots: Tollip. SYBR Green, 03-04-2009, 441 project 1st run.mxp. Dissociation Curve: Tollip.

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Methods

Enumeration of Bacteriophage and Prokaryotic Populations in an Urban Coastal Wetland, the Ballona Wetlands, by Confocal Microscopy. . Methods. Collection. 1 sample collection from the Ballona Wetlands during the ebb flow 1 sample collection during the flood flow

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qPCR SNAPSHOTS LIVE

qPCR SNAPSHOTS LIVE. MAY 13, 2003. Agenda. Introduction Objectives & Methodologies Detection Chemistries Instrumentation Conclusion. Real-Time PCR. Ability to detect PCR product as it is synthesized

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Primer & Probe Design Affects Ability to Detect and Quantify Variant Viruses

Primer & Probe Design Affects Ability to Detect and Quantify Variant Viruses. Sally Baylis, Piotr Grabarczyk & Jacqueline Fryer. Detection of Parvovirus B19 Genotypes with SYBR Green. A6 a t t c g ca a

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