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Electrochemical Detection in HPLC

Electrochemical Detection in HPLC. ALEXYS ™ - HPLC/ECD. Applications Neuroscience Pharma Clinical analysis Food & Beverages Environmental. Why ECD?. extremely sensitive highly selective . Principle of HPLC/ECD. electrochemical reaction detector potential (E) is driving force.

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Electrochemical Detection in HPLC

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  1. Electrochemical Detection in HPLC

  2. ALEXYS™ - HPLC/ECD • Applications • Neuroscience • Pharma • Clinical analysis • Food & Beverages • Environmental

  3. Why ECD? • extremely sensitive • highly selective

  4. Principle of HPLC/ECD • electrochemical reaction detector • potential (E) is driving force

  5. Electrochemical reaction ΔE diffusion electron transfer reaction

  6. 100 80 60 Signal (%) 40 20 0 0 400 800 1200 1600 E (mV) Role of electrode potential E • Facilitates the EC reaction oxidation occurs no reaction A  B

  7. Working range potential E • E working range determined by mobile phase

  8. Peak height vs. concentration • UV  Beer’s law E =  d C • ECD  Cottrell equation ilim= 1.47 n F (Fm )1/3 (DA/b)2/3 C A= WE area A b = spacer thickness C = concentration ilim= diffusion limited current measured (peak height) peak height (E) vs concentration (C) peak height (Ilim) vs concentration (C)

  9. Peak height vs. concentration • UV  Beer’s law E =  d C • ECD  Cottrell equation ilim= f (A/b)2/3 C A= WE area A b = spacer thickness C = concentration ilim= peak height as simple as abc…

  10. Electroactive Groups

  11. Application areas… www.myAntec.com

  12. Summary Electrochemical Detection for HPLC • Potential driven • Selective • Sensitive Thank you!

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