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This document explores the process of gene expression, detailing how DNA is transcribed into mRNA and then translated into proteins. Key processes like transcription and translation are outlined, including steps like RNA polymerase binding to the promoter, nucleotide pairing, and the role of tRNA in amino acid transfer. It also examines the genetic code, showing how codons relate to amino acids and highlighting the significance of mutations such as deletion, duplication, inversion, and translocation in genetic expression.
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I. Genes to proteins p. 208 – Gene expression A. Decoding DNA 1. RNA: ribonucleic acid
B. Transfer of DNA into protein – p. 208 1. transcription – 2. translation –
C. Steps in transcription pg 209 1. RNA polymerase binds to a promoter 2. Separation of the two strands 3. Complementary RNA nucleotides are added 4. mRNA – is released out of the nucleus
D. Steps of Translation pg 211 1. mRNA enters the cytoplasm 2. tRNA pairs up with mRNA with the help of rRNA pg 212 a. nucleotides are read 3 at a time codon pg 211 anticodon pg 212
3. tRNA transfers its amino acid to the mRNA train 4. Amino acids are added to a chain until tRNA hits a stop codon
What would be the amino acid sequence for this mRNA strand? AUG/GGG/CGG/AAG/AAA/UAG
AUG/GGG/CGG/AAG/AAA/UAG “start”/Glycine/Arginine/Lysine/Lysine/”stop”
DNA: TAC/CCT/GGC/ACT mRNA: COMPLEMENTARY CODON BONDING in Protein Synthesis:
DNA: TAC/CCT/GGC/ACT mRNA: AUG/GGA/CCG/UGA tRNA:
mRNA: AUG/GGA/CCG/UGA tRNA: UAC/CCU/GGC/ACU Amino Acids:
mRNA: AUG/GGA/CCG/UGA tRNA: UAC/CCU/GGC/ACU Amino Acids: “start”/glycine/proline/”stop”
mRNA: AUG/GGA/CCG/UGA tRNA: UAC/CCU/GGC/ACU Amino Acids: “start”/glycine/proline/”stop”
III. Mutations • Dleletion - • Duplication- • Inversion - • Translocation-