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BioBrick Fusion Complex Expression and Dimerization Study

This study focuses on assembling toxin receptor fusion complexes to determine if they bind and undergo dimerization. Expression is tested using various promoters such as R0040 and B0030. Fluorescein dimer binding to scFV and cells is also investigated. The actuator ctx and GFP J07011 are utilized to study fluorescein binding signals. The functional aspects of the fusion complex expression and dimerization are analyzed.

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BioBrick Fusion Complex Expression and Dimerization Study

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  1. Actuator Input Input Head Receiver Receiver/ Transmitter 1: ToxR Assemble: J07027,J07028 prom::rbs::toxR::MalE/PhoA BioBrick Pho A: J07005 MalE: J07005 Receive Brick ToxR: J07009 Can Fluor. Bind to fusion? Does fluor. bind ⇨⇨ dimerization? Is Fusion Complex Being Expressed? Where Is Fusion Complex Being Expressed? Assemble: R0040::B0030 prom::rbs Assemble: J07024-26 prom::rbs::toxR::scfv BioBrick anti-fluor scFV J070012, J070013, J070014 Test expression of scFV prom::J070012, J070013, J070014 Fluorescein dimer binds to scFV? Fluorescein ⇨ Cells? Fluorescein dimer ⇨Cells? Actuator: ctx::gfp J07011 Fluorescein Binding ⇨⇨ signal? Receive Brick ctx: J07007 Dimerization ⇨⇨ signal?

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