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Experimental Protocol for Assessing Vascular Responses in Rats Under Dietary Changes

This study involves 12 rats (5 males, 5 females, 2 parental) maintained on a Purina 5001 diet, aged 8 weeks. Over a two-week period, body weight, body temperature, and respiratory parameters are monitored. After 10 days on a high-salt diet (4% NaCl), 24-hour urine collections are conducted using metabolic cages, followed by ultrasound assessments and blood sampling for vascular protocols. The experiment aims to evaluate vascular relaxation and contraction responses to dietary changes while assessing kidney and heart weights.

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Experimental Protocol for Assessing Vascular Responses in Rats Under Dietary Changes

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  1. Barrier (animal production) 5M/5F KO, 2M parental Holding (Purina 5001 diet) Friday 12 rats @ 8 weeks old Movement protocol Body weight, body temperature Movement, breathing freq (TI, TE) CO2 challenge Calibrate, body weight, temp Friday – Monday Monday AM 12 rats Monday PM Conditioning Change diet to 4% NaCl (Dyets) Maintain HS diet for 2 weeks 24 hour urine collection; non-fasted; (Th→F, day 10 HS) Wednesday Monday 4 rats 4 rats Tuesday 4 rats Urine collection Metabolic cages, fast, overnight urine collection Urine collection Metabolic cages, fast, overnight urine collection Urine collection Metabolic cages, fast, overnight urine collection Thursday AM Tuesday AM Wednesday AM Ultrasound Body weight, ultrasound, MAP, blood sample, aorta for vascular protocol, kidney weight, heart weight, DNA tissue Ultrasound Body weight, ultrasound, MAP, blood sample, aorta for vascular protocol, kidney weight, heart weight, DNAtissue Ultrasound Body weight, ultrasound, MAP, blood sample, aorta for vascular protocol, kidney weight, heart weight, DNA tissue Thursday PM Tuesday PM Wednesday PM Vascular Relaxation & contraction vascular protocol Vascular Relaxation & contraction vascular protocol Vascular Relaxation & contraction vascular protocol

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