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Lab Exercise 14

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Lab Exercise 14

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    1. Lab Exercise 14 Gram Staining

    2. Background Gram Staining developed in the late 1800s Gram Staining is a Differential staining technique that distinguishes between 2 broad groups of bacteria based on differences in the cell wall.

    4. Staining Procedure Place a slide with a bacterial smear on a staining rack. STAIN the slide with crystal violet for 1-2 min. Pour off the stain. Flood slide with Gram's iodine for 1-2 min. Pour off the iodine. Decolourize by washing the slide briefly with acetone (2-3 seconds). Wash slide thoroughly with water to remove the acetone - do not delay with this step. Flood slide with safranin counterstain for 2 min. Wash with water. **Gram Stain Animation

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