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This study presents Supplementary Figure 1, illustrating the complex dynamics of inversion and excision events at various genetic sites, specifically F3 and lxP. Included are details on trapped alleles, inverted alleles, and re-inverted alleles, showing the interactions between different genomic elements. The roles of FLP and Cre recombinases in mediating these processes are discussed. Additionally, the figure highlights inversion events at E1, E2, and SA sites, providing insights into the implications for allele behavior and potential applications in genetic engineering.
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Supplementary Figure 1 frt F3 frt F3 lx5171 lxP lxP lx5171 E1 OPE OPE OPE OPE OPE OPE E2 pA SA bgeo pA inversion at F3 sites inversion at frt sites E1 E2 pA OPE OPE OPE OPE OPE OPE SA bgeo pA Inversion by FLP E1 OPE OPE OPE OPE OPE OPE E2 pA pA bgeo SA excision at frt sites excision at F3 sites E1 pA bgeo SA E2 pA inversion at lxP sites inversion at lx5171 sites SA bgeo pA E1 E2 pA Re-inversion by Cre SA bgeo pA E1 E2 pA SA bgeo pA E1 E2 pA excision at lx5171 sites excision at lxP sites Trapped allele Inverted allele Re-inverted allele
