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Genomic Characterization and Targeted Allele Design for LacZ Expression in Research Models

This document outlines the design and characterization of a targeted allele incorporating LacZ for gene expression studies. The allele utilizes a combination of targeting vectors, including pA sequences for efficient transcription and selection markers like Neo for positive selection. Detailed descriptions of the constructs, such as the 5’ and 3’ probes, and their integration using loxP and frt sites are provided. This research aims to facilitate studies on gene function and regulation in specified laboratory settings.

evangeline-phelps
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Genomic Characterization and Targeted Allele Design for LacZ Expression in Research Models

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  1. 2kb 遺伝子名 研究室 担当 XXkb Wt allele 3’ Probe 5’ Probe Targeting vector LacZ pA Pr Neo pA Pr DT-ApA lox71 frt frt loxP Targeted allele LacZ pA Pr Neo pA 3’ Probe 5’ Probe YYkb LacZ pA Pr Neo pA

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