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IDENTIFYING MACROMOLECULES IN Nutrient LAB

IDENTIFYING MACROMOLECULES IN Nutrient LAB. AICE Biology. In this lab, with the use of indicators as chemical detection tools, you will analyze a variety of foods for the presence of nutrients. Detection is based upon observing a chemical change that takes place most often a change in color. .

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IDENTIFYING MACROMOLECULES IN Nutrient LAB

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  1. IDENTIFYINGMACROMOLECULES IN NutrientLAB AICE Biology

  2. In this lab, with the use of indicators as chemical detection tools, you will analyze a variety of foods for the presence of nutrients. Detection is based upon observing a chemical change that takes place most often a change in color.

  3. Lab Objective Identify the presence of major nutrients such as simple carbohydrates (glucose), complex carbohydrates (starch), protein, sodium, vitamin C and fat in common foods.

  4. What is an indicator? • Indicators are chemical compounds used to detect the presence of other compounds.

  5. Background Information

  6. Test for Simple CarbohydratesBenedict’s solution • Benedict's solution is a chemical indicator for simple sugars such as glucose: C6H12O6. • Aqua blue: negative test; yellow/green/brick red, etc.: positive test

  7. Test for Simple CarbohydratesBenedict’s solution • Unlike some other indicators, Benedict’s solution does not work at room temperature - it must be heated first.

  8. Test for Complex CarbohydratesIodine Solution • Iodine solution  color change = blue to black

  9. Test for Complex CarbohydratesIodine solution • Iodine solution is an indicator for a molecule called starch. • Starch is a huge molecule made up of hundreds of simple sugar molecules (such as glucose) connected to each other.

  10. Your turn. • In your notebook, make a data table like that shown below, using four different foods. Next make predictions, guessing what macromolecules each food contains. Mark + for positive, - for negative and +/- for foods that appear to have a small amount of the macromolecule.

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