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Seeding

Seeding. Terese Bergfors Dept of Cell and Molecular Biology Uppsala University, Uppsala Sweden terese@alpha2.bmc.uu.se Examples of two new structures from our laboratory where seeding was critical to obtaining the crystals. Seeding. Why seed? How? What to use as a seed?.

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Seeding

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  1. Seeding Terese Bergfors Dept of Cell and Molecular Biology Uppsala University, Uppsala Sweden terese@alpha2.bmc.uu.se Examples of two new structures from our laboratory where seeding was critical to obtaining the crystals

  2. Seeding • Why seed? • How? • What to use as a seed?

  3. Some methods for optimization microgravity seeding

  4. Seeding and the phase diagram Luft and De Titta, Acta Cryst. (1999) D55, 988-993

  5. Serial seeding 20 mg/ml 10 mg/ml 10 mg/ml

  6. What to seed from? oil Take your best crystal or any solid phase

  7. Streak seeding whisker Touch crystal Draw streak line Enrico Stura in “Crystallization of Nucleic Acids and Proteins” Ed. Ducruix and Giege, 1992 Oxford University Press p. 108

  8. Does seeding solve everything? before after

  9. After Seeding, Ni Does seeding solve everything? After seeding, Ni, and ligand

  10. Summary of Tips • Lower concentration • Use any solid phase • Serial seed • Use sitting drops

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