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Mapping for Marker Assisted Selection

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Mapping for Marker Assisted Selection

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    1. Mapping for Marker Assisted Selection

    3. Genetic Mapping U.S. germplasm Beauregard x Tanzania Beauregard x Wagabolige 2nd generation linkage map AFLP, ISSR, microsatellites Anchor loci Marker assisted breeding Quality traits Resistances

    4. Genetic Mapping Beauregard Orange fleshed Susceptible Short growth period Tanzania and Wagabolige Pale flesh color SPFMV resistant Highly RKN resistant Long growth period

    5. Genetic Mapping

    6. Parental Genotypes

    18. Quantitative Traits Polygenic inheritance Continuous gradient Normal distribution Yield, sugars, starch etc. Additive and dominance effects Interval analysis Single point analysis

    19. QTL analysis QTL analysis using WinQTL cartographer Proc CORR (SAS v9.1) Proc GLM (SAS v9.1) Framework map based on SD AFLP markers Drawings in MapChart v2.1

    21. QTL analysis QTL analysis using WinQTL cartographer Proc CORR (SAS v9.1) Proc GLM (SAS v9.1) Framework map based on SD AFLP markers Drawings in MapChart v2.1

    22. RKN Linkage group T41

    23. QTL for RKN resistance

    24. QTL for RKN resistance (contd)

    26. QTL for RKN resistance

    27. Bacterial artificial chromosomes Genomic libraries Physical mapping Large insert sizes up to ~150-200kb. Low copy Big plasmids

    29. Physical mapping

    31. Peach mapping General Prunus map Includes peach, almond, apricot Nearly 1000 molecular markers Over 2200 mapped ESTs (genes) Sosinski lab contributed 400 Physical mapping underway DOE funded peach genome sequencing

    32. Marker assisted selection

    35. Comparative Genomics

    36. Comparative Genomics

    37. Comparative Genomics

    38. Comparative Genomics

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