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Data Blitz

Data Blitz. Eric Spivey 30 March 2011. Biopolymer Solution. Background: Using pulsed laser-generated multiphoton excitation to fabricate micron scale hydrogel matrices from biopolymer solutions. Biopolymer Hydrogel Matrix. Focused, pulsed laser. Microscope Objective.

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Data Blitz

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  1. Data Blitz Eric Spivey 30 March 2011

  2. Biopolymer Solution Background: Using pulsed laser-generated multiphoton excitation to fabricate micron scale hydrogel matrices from biopolymer solutions. Biopolymer Hydrogel Matrix Focused, pulsed laser Microscope Objective Motivation: Use this method to fabricate cell scaffolds for neural tissue engineering applications. Goal:Fabricate hydrogel microstructures with tunable functional and mechanical properties.

  3. Multiphoton fabricated PEG-DA hydrogels • Why PEG? • FDA approved biomaterial, non-immunogenic • Widely used in tissue engineering studies as a hydrogel • Widely used in drug delivery for tunable release properties • Issues • Not naturally degradable • Tricky solubility in aqueous solutions at low MW • Fairly strong negative charge

  4. poly(ethyleneglycol) diacrylate (PEGDA, average mw ~700 Da) PEG structures =13 25 µm 25 µm

  5. Fabricating PEG structure using a Mask

  6. Using ESEM

  7. Functionalization of PEG with BP-biotin 2 mW 3mW 4mW 5 mW

  8. Indentation Modeling Force = F Radius R Indentation = d • Can calculate Young’s Modulus with F, R and d, • Assume: • Poisson’s ratio is 0.5 and • Substrate measured is infinitely thick • Corrections exist for non-infinite substrates

  9. Setup for Validating Finite Thickness Model Δh ~8 µm Glass PVA Force Map (3x)

  10. Validation of finite-thickness Hertz Model

  11. BSA+GMHA Modulus(20% BSA w/v)

  12. PEG + GMHA Modulus(60% PEG v/v)

  13. PEG + GMHA Modulus(30% PEG v/v)

  14. NG 108 cells in PEG structures

  15. Questions?

  16. Talk on Thursday  "Neuroscience on a Chip" Albert Folch Professor, Department of Bioengineering University of Washington Thursday, March 31, 2011 at 3:30 PM NHB 1.720

  17. Stage Light Effect

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