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This study investigates the temporal dynamics of TNF (Tumor Necrosis Factor) and its downstream signaling proteins, including p65, and their interactions within a cellular context. Using a combination of medium treatments and various time points, we explore the effects of TNF on cell signaling pathways, with evaluations using assays for p-p65 (Ser536) and PARP, as well as loading controls such as b-actin. This comprehensive analysis aids in understanding the regulatory mechanisms of inflammation and apoptosis in response to TNF.
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A Control 40X 40X MA TNF 40X TNF+MA 40X p65 DAPI Merge Medium MA 0 5 15 30TNF time (m) 0 5 15 30 B p65 NE p-p65 (Ser536) PARP CE p65 b-actin C Medium MA 0 5 10 20 40 0 5 10 20 40TNF time (m) IkBa b-actin