Perfused Bioreactor with Matrix-Enabled Capillary Scaffold (MECS) Team Members: Allyson Fry Bryan Gorman Jonathan Lin William Wong Advisor: Dr. John P. Wikswo A continuation from 2004-2005 (Barnett, Garrett, Harvill, Mayer, McClintock)
Objective Design a tissue engineering scaffold and supporting microfluidic control system to guide the development of a network of capillary tubes in vitro.
Introduction • Bioreactor • Capillary network • Matrix-Enabled Capillary Scaffold (MECS) • Perfusion
Motivation • Simplified model for disease research • Human system • Control at many levels • Variety of uses http://www.kids4research.org/mouse%20in%20hand%20facing%20left.jpg
Martins-Green a a a a
Bioreactor Design Supply Network Support Filter Collagen MECS Collagen Support Filter Supply Network
Use two-layer photolithography to create mold of ‘egg crate’ design Use soft lithography to create a PDMS stamp Use stamp to deposit a sacrificial layer between two layers of collagen Fabrication Strategy
Main Control Program Histories of graph values Graphs pH ranges PID coefficients pH sensor settings Pump settings
Project Goals • Model a complete microvascular network • Support cellular processes • Allow evaluation of molecular mechanisms • Provide environment for endothelial cell differentiation • Facilitate formation of stable tubular structures • Support a flow of perfusate
References • Martins-Green M, Li Q, Yao M. A New Generation Organ Culture Arising from Cross-Talk Between Primary Human Cell Types. Unpublished. • Nanney, Lillian et al. Unpublished research. • Tang MD et al. Fabrication of collagen gels that contain patterned micrometer-scale cavities. Adv. Mater, 2004.