ERT314 BIOREACTOR SYSTEM CHAPTER 2: BIOLOGICAL SYSTEMS AND MEDIA DESIGN
**rheology – the study of the flow of matters, especially in liquid state
Fermentation with fungi Fermentation with bacteria
Oxygen Demand • OTR = Oxygen transfer rate • OUR = Oxygen uptake rate
E.Coli has a system where the capacity to use these precursors exceed the capasity of intermediary metabolism e.g: glycoprotein, oligosaccharides, enzyme etc.
2.2.1 Microbial ProcessesA. Design and Formulation of Media Should consider:
e.g: e.g: antagonism (Zn+ to Cu2+), synergism (toxic Cu2+ + Cd2+), stimulation (Ca2+ and Mn2+ depends on Zn2+)
requires higher pantothenic acid content in media Addition of glycine betaine in media for L- amino acid production which has high glucose concentration, acts as an osmotic protectant.
causing Whereas others, like dried yeast are generally uniform from batch to batch.
Where yx/s, yp/s and yCO2/s are = coefficients related to biomasss, product and CO2 formation = expressed as C mole of biomass, product and CO2 per mole of carbon and energy source (substrate) σi = carbon fraction of i (x,p,s) Yx/s = g dry weight of biomass per g of substrate consumed Yp/s = g dry weight of product per g of substrate consumed
Constraints: • Low reactor rated and productivity • Instability of cell lines presents problem not easy to overcome • Danger of microbial contamination • Secondary metabolites is often retained in biomass • Plant cells are often shear sensitive • In some cases, secondary compounds should be removed rapidly How to overcome?