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Tissue Bioreactor

Tissue Bioreactor

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Tissue Bioreactor

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  1. Tissue Bioreactor by Rachel Mosher, Joel Gaston, Kara Barnhart Client: Susan Thibeault Advisor: Brenda Ogle (October 19, 2007)

  2. Outline Stimulating Vocal Fold Environment Vocal Folds Bioreactor Background Vibratory and Operational Requirements Rotation Problem Solution Ideas Assembly, Cell Culture, & Testing Conclusion

  3. Problem Statement Realistic simulation of vocal fold environment Improve Previous Design: T-flask needs to be leak-free Motors need to be programmable Multiple stimuli required Tecoflex substitute

  4. Background – Vocal Folds 2 mucous membranes Extracellular matrix Cartilage Horizontally stretched across larynx Vibration ranging 0-400Hz Modulate airflow from lungs during phonation

  5. Background – Vocal folds

  6. Bioreactor Background • Supports/Mimics a biological system • Similar to in vivo environment • Fibroblasts cultured on cellular substrate • Fluid provides nutrition • Research on healthy and injured vocal fold tissue

  7. Evolution of the Bioreactor Built by Hitchcock/Titze Vibration and tensile stress Cell culturing/seeded Tecoflex strips Relatively simple design

  8. Evolution of the Bioreactor BME design Spring 2007 Vibration Problems Leakage New substrate No support for vibration bar

  9. Evolution of the bioreactor • Work over the summer • Address design issues/flaws, client requirements • Research • Design • Parts Ordering • Machining • Much more work to be done

  10. Evolution of the Bioreactor Current design Quality materials Vibration, tension, rotation between angles New and improved cellular substrate

  11. Client Requirements Concerted bar vibration (50-400Hz frequency) Contact between strips during vibration Easy to sterilize and/or having disposable parts Tecoflex substrate alternative

  12. Design Ideas Moment reaction due to motion of actuator Pneumatic System Bearing/Wheel Magnetic Repulsion Suspension by String/Wire

  13. Pneumatic System Cons • Brings outside material into system, disturbing stability • Difficult to construct and implement • Not cost effective Pros No Friction

  14. Bearing/Wheel Pros • Cost Effective • Simplistic • Easy to construct and apply Cons • Some friction • Induces wear and tear on the system Polypropylene Tubing Bearings Actuator support

  15. Magnetic Repulsion Side View Back View Polystyrene Tubing Actuator Support Magnets Pros Frictionless Highly cost effective Fairly easy to construct and implement Cons Magnetic fields will affect electronics Magnetic fields may affect cells

  16. Suspension System Suspension Wire Polypropylene Tubing Copper Tubing Pros Simple Highly cost effective Minimal effect on the rest of the system Easy to construct Cons Produces friction Unknown problems may arise; could interfere with vibrations

  17. Design Matrix

  18. Future Work Ordering custom parts Fabricating needed parts Assembling the bioreactor Culturing vocal cord cells Glycosan material Testing and Analysis Redesigning

  19. Conclusion Research vocal folds Proper stimuli essential Suspension of moving parts to prevent undesirable rotation Parts need ordering and fabrication Testing with cells

  20. Credits Brenda Ogle Susan Thibeault Stephanie Bartley Glenn Prestwich