1 / 12

Use of Brilliant Blue G to Improve Recovery After a Spinal Cord Injury

Use of Brilliant Blue G to Improve Recovery After a Spinal Cord Injury. Administration of an antagonist of the ATP-sensitive receptor P2X 7. Results of Spinal Cord Injury. Excess of Extracellular ATP Activation of P2X7 acceptors Change in astrocytes Motor neuron death.

addison-pennington
Télécharger la présentation

Use of Brilliant Blue G to Improve Recovery After a Spinal Cord Injury

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Use of Brilliant Blue G to Improve Recovery After a Spinal Cord Injury Administration of an antagonist of the ATP-sensitive receptor P2X7

  2. Results of Spinal Cord Injury • Excess of Extracellular ATP • Activation of P2X7 acceptors • Change in astrocytes • Motor neuron death

  3. Effects of Extracellular ATP • 1 Treatment– No Change • 2 Treatments – 27% Degradation • 3 Treatments – 36% Degradation

  4. Comparison of ATP to Degradation products of ATP

  5. Activation of P2X7 Through Other Means • P2X7 agonist 2',3'-O-(4-benzoylbenzoyl)ATP(BzATP) used to activate P2X7 • 30% motor neuron attrition

  6. Inhibition of P2X7 • Based on previous experiments using P2X7 agonists, researchers posited the use of an antagonist would block the phenotype change in the astrocytes. • OxATP • Brilliant Blue G(BBG) • Also known as FD&C Blue #1

  7. Lab rats paralyzed by dropping a 10 gram weight from a height of 12.5 mm onto the spinal cord • Three test groups • Vehicle • 10 mg/kg • 50 mg/kg

  8. Surprising Results • Treatment with BBG for 3 consecutive days • Regained ability to walk • Turned Eyes and Skin Blue!

  9. Results at 42 Days BBG Treated Vehicle Treated • Movement of hind legs • Coordinated movement of front and rear legs • Ability to support plantar steps • Movement of hind legs • Not coordinated • Could not support plantar steps

  10. Comparing results Untreated Treated

More Related