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Announcements

Announcements. Exam 1 scores and key will be posted by tomorrow Key terms 4 now posted on website Homework 4 will be posted by tomorrow; due next Wednesday. Exam 1 results. With 5 pt. curve. Before curve. Average: 79.8. Average: 74.8. BIO 304 Lecture 11.

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Announcements

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  1. Announcements Exam 1 scores and key will be posted by tomorrow Key terms 4 now posted on website Homework 4 will be posted by tomorrow; due next Wednesday

  2. Exam 1 results With 5 pt. curve Before curve Average: 79.8 Average: 74.8

  3. BIO 304 Lecture 11

  4. What kind of experience do you have with molecular biology methods (restriction digests, agarose gels, plasmid DNA, etc.)? 1. Totally new subject for me 2. I've learned about it in class but have no hands-on experience 3. I've done some of this in my high school or college Biology classes 4. I've gotten a lot of experience from a BIO395 or other independent research project

  5. Recombinant DNA technology: what’s the point?

  6. Recombinant DNA technology: what’s the point? Unit of inheritance The Gene Unit of function Unit of variation

  7. What I’m assuming you already know…

  8. A single strand of DNA has the sequence 5'-TGCATTCCAG-3'. Its complementary strand must have the sequence 1. 5'-TGCATTCCAG-3' 2. 5'-ACGTAAGGTC-3' 3. 3'-TGCATTCCAG-5' ✓ 4. 5'-CTGGAATGCA-3'

  9. Formation of a recombinant DNA molecule

  10. Restriction enzymes sticky ends blunt ends

  11. Ligation of two different DNA fragments

  12. Cloning vectors

  13. Introduction of recombinant DNA into bacteria

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