1 / 7

Nicholas lab: C. elegans research

Nicholas lab: C. elegans research. Working with worms Postgraduate induction 2013 Yee Lian Chew. C. elegans – the roundworm. Small – 1 mm long in adults Anatomy has been fully mapped Highly stereotypical

arch
Télécharger la présentation

Nicholas lab: C. elegans research

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Nicholas lab: C. elegans research Working with worms Postgraduate induction 2013 Yee Lian Chew

  2. C. elegans – the roundworm • Small – 1 mm long in adults • Anatomy has been fully mapped • Highly stereotypical • Reproduce sexually by mating with males and also by self-fertilisation (maintained as a haermaphrodite population) • Short-lived • Transparent

  3. What we do Hong Anna Aaron Hannah Sashi Duygu Matthew Yee Lian Slavica Priya Estelle Irene Mallory

  4. C. elegans – techniques we use • Generation of transgenic lines – biolistic transformation and microinjection • Molecular biology techniques including DNA/RNA work, qPCR, RNAi • Staining, western blot, mass spec (iTRAQ) • Microscopy – EM, confocal, fluorescence, dissecting scope • Dissections – germline staining • And of course, lots of fun with worms

  5. Worms are transparent – and yes, they have brains They have 302 neurons

  6. Co-localisation studies using transgenic C. elegans pdpy-7::HIS-24::mCherry pctbp-1::ctbp-1::gfp Merge Matthew Hoe

  7. Any questions? • Contact the boss: hannah.nicholas@sydney.edu.au

More Related