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Dive into the fascinating world of microbiology, exploring microscopic organisms including bacteria, fungi, algae, protozoa, and viruses. While only a small percentage are pathogenic, all require essential resources like water, nutrients, and appropriate temperatures for growth. Learn about bacterial reproduction through binary fission, conjugation, and transformation, as well as the diversity of shapes and arrangements. Also, discover historical theories of disease and key contributors to germ theory, including Pasteur and Koch. Uncover how microbes impact our world in profound ways.
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MICROBIOLOGY STUDY OF BACTERIA & VIRUSES
WHAT ARE MICROBES? • Microscopic organisms that include bacteria, fungi, algae, protozoa & viruses • Less than 5% are classified as pathogenic (disease causing)
WHAT DO M/O’S NEED? • Water • Food/nutrients • Oxygen • Aerobes, anaerobes, facultative anaerobes • Temperature • Psychrotrophic (32-45 F) • Mesophilic (60-110 F) • Thermophilic (110-150 F) • pH • 7 optimal • Reproduce 4.5 - 10
REPRODUCTION • Bacteria • Binary Fission • 1 bacteria clone itself • Produce 2 identical cells • Conjugation • Two bacteria share genetic information • Produce 2 new cells • Transformation • Bacteria takes genes from environment
REPRODUCTION • Molds • Fragmentation • Pieces break off & create new individual • Budding • Copy (similar b. fission) • New copy break off of original • Spores • Reproductive cells released either free-form or join w/ another
BACTERIA • Prokaryotic cells • No nucleus • Contain genetic material plasmid • Single round chromosome • Ribosomes –make proteins • Pilli • protein hair-like structures • Help cells attach • Flagella • movement
BACTERIAL STRUCTURES • Cell Wall • Rigid structure either carbs or proteins • Prevents osmotic pressure (cytolysis) • Capsule • Sticky gelatin surrounds c.wall • Prevents wbc from attaching • Outer Membrane (some forms) • Toxic lipid bilayer • Endospore(some forms) • Hard outer covering • Prevents drying out • Allows go dormant
MOLD • Eukaryotic Cells • Cross between plant & animal cell • Cell walls • No Chloroplasts • Consumers saprophyte (absorb nutrients from dead, decaying organisms)
MOLD • Multi-cellular organisms composed of branches called “hypae” • Vegetative hypae attached to host • Aerial hypae absorb O2 from air
M/O IN ENVIRONMENT LAB • What area of EPHS will support the most amount of microbial growth? • 20 Possible locations: • - • - • - • - • - • - • - • - • - • - • - • - • - • - • - • - • - • - • - • - • - Control
BACTERIAL SHAPES • Cocci (coccus) • Round cells • Bacilli (bacillus) • Rod shaped • Spirella (spirellum) • Thick, rigid spiral • Spirochete • Thin, flexible spiral • Vibrio • Curved, comma shaped
BACTERIAL ARRANGEMENTS • Diplo • Arranged in pairs • Diplococci, diplobacilli • Strepto • Arranged chains • Streptobacilli • Staphlo • Arranged in clusters • staphlococcus
THEORIES ON DISEASE • Prior to physical evidence of m/o’s • Common beliefs witchcraft, sins against God • Lucretius (Roman philosopher) • Published theories form of poem • Renounced idea of gods/spirits • Fracastoro • Theory of chemical “spores” travel great distances • Leeuwenhoek • Credited w/ discovering bacteria • Looking a plaque family member’s teeth
GERM THEORY OF DISEASE • Bassi • Discovered silkworm disease caused by a fungus • Killing caterpillars & destroyed silk industry • Berkeley • Proved Great Potato Blight caused by a fungus
GERM THEORY OF DISEASE • Lister • Developed system antiseptic surgery • Combination heat & phenol alcohol • Koch • Developed criteria proving relationship btw m/o’s & disease • Studied anthrax • Koch’s postulates • M/o found all cases disease • Maintained pure culture • Produce original infection, after generations in culture • Isolated from inoculated host & recultured
GERM THEORY OF DISEASE • Pasteur • Developed 1st vaccine for Rabies • Used attenuated strain (lost ability cause disease) • Gram • Developed system of staining procedures • Divides bacteria 2 grps • Gram + turn violet • Cell walls simple carbs • Less pathogenic • Gram - turn red • Cell walls protein & extra lipid outer membrane • Resistant antibiotics • Pathogens